| 用精子载体法将MT-hGH基因导入泰山赤鳞鱼 |
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| 引用本文： | 翟玉梅,鹿培源,崔博文,王喆,时永香,杨晓梅,赵晶,岳永生.用精子载体法将MT-hGH基因导入泰山赤鳞鱼[J].海洋与湖沼,2001,32(1):37-41. |
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| 作者姓名： | 翟玉梅 鹿培源 崔博文 王喆 时永香 杨晓梅 赵晶 岳永生 |
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| 作者单位： | 1. 山东大学生命科学学院
2. 山东农业大学动物科技学院 |
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| 基金项目： | 山东省科学技术委员会攻关项目!D97060号。 |
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| 摘 要： | 于1998年3月－1999年2月，在山东农业大学采集泰山赤鳞鱼，采用精子载体法进行MT－hGH基因导入的研究。将泰山赤鳞鱼的精子在保存液内与含有MT－hGH基因的重组质粒保温30min，然后用于人工授精，共孵化出363尾幼鱼。待鱼苗长至5cm左右时，对其中的39尾进行PCR和Southern杂交检测。结果表明，有7尾鱼苗带有MT－hGH基因,转基因鱼的阳性率为17．95％。
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| 关 键 词： | 精子载体法 基因转移 泰山赤鳞鱼 MT－HGH基因 转基因鱼 |
| 收稿时间： | 1999/7/12 0:00:00 |
| 修稿时间： | 1999年7月12日 |
TRANSFERING MT-hGH GENE INTO VARICORIHINUS TRACROLEPIS WITH SPERM AS VECTORS |
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| ZHAI Yu-mei,LU Pei-yuan,CUI Bo-wen,WANG Zhe,SHI Yong-xiang,YANG Xiao-mei,ZHAO Jing and YUE Yong-sheng.TRANSFERING MT-hGH GENE INTO VARICORIHINUS TRACROLEPIS WITH SPERM AS VECTORS[J].Oceanologia Et Limnologia Sinica,2001,32(1):37-41. |
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| Authors: | ZHAI Yu-mei LU Pei-yuan CUI Bo-wen WANG Zhe SHI Yong-xiang YANG Xiao-mei ZHAO Jing and YUE Yong-sheng |
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| Institution: | College of Life Sciences, Shandong University, Jinan, 250100;College of Life Sciences, Shandong University, Jinan, 250100;College of Life Sciences, Shandong University, Jinan, 250100;College of Life Sciences, Shandong University, Jinan, 250100;College of Life Sciences, Shandong University, Jinan, 250100;College of Life Sciences, Shandong University, Jinan, 250100;College of Life Sciences, Shandong University, Jinan, 250100;College of Animal Science, Shangdong Agriculture University, Taian, 271018 |
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| Abstract: | Transfering MT-hGH gene into Varicorhinus tr acrolepis with sperms as vectors was carried out from March, 1998 to February, 1999. The Varicorhinus tracrolepis sperms were incubated with MT-hGH gene i n dilute citrate solution for 30 minutes. Then they were used in artificial inse mination. 997 eggs were inseminated with the treated sperms and 755 of them were fertilized. 363 fish were hatched in 3-4 days. When the fish grew to about 5cm , genomic DNA was isolated from 39 fish. The samples were tested by PCR and Sout hern blot hybridization. The result shows that 7 fish contained MT-hGH gene. The positive rate was about 17.95%. |
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| Keywords: | Sperm vectors Gene transfer Varicor hinus tracrolepis MT-hGH gen\ |
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