| 脊尾白虾(Exopalaemon carinicauda)3 个野生群体mtDNA 16S rRNA 序列差异及长臂虾科系统进化关系 |
| |
| 引用本文： | 马 朋,刘 萍,李 健,李吉涛,高保全.脊尾白虾(Exopalaemon carinicauda)3 个野生群体mtDNA 16S rRNA 序列差异及长臂虾科系统进化关系[J].海洋与湖沼,2012,43(1):174-179. |
| |
| 作者姓名： | 马 朋 刘 萍 李 健 李吉涛 高保全 |
| |
| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,青岛266071／大连海洋大学,大连116023
2. 中国水产科学研究院黄海水产研究所,青岛,266071 |
| |
| 基金项目： | 国家高技术研究发展计划项目资助, “主要养殖甲壳类良种培育”, 2012AA10A409 号; 山东省科技发展计划项目资助，2011GHY11526 号; 农业科技成果转化资金项目资助, 2010GB23260589 号 |
| |
| 摘 要： | 采用PCR扩增和序列测定等技术,对脊尾白虾3个野生群体共计58个个体的线粒体16SrRNA基因片段进行初步研究。结果表明,在获得的长度为509bp核苷酸序列中,共检测到7个变异位点、8种单倍型,除象山湾群体外其它两个群体遗传多样性水平都较低。AMOVA分析表明,象山湾与莱州湾群体有显著的遗传差异,其余群体间的遗传差异不显著。另外,将本研究所得序列与GenBank中长臂虾科11个种的16SrRNA基因片段序列进行比较后,发现其聚类关系与传统分类略有不同,并依据16SrRNA序列变异百分比推测了长臂虾科12个种的大致分化时间。
|
| 关 键 词： | 脊尾白虾 16S rRNA基因 遗传多样性 系统进化 |
| 收稿时间： | 2010/12/25 0:00:00 |
| 修稿时间： | 2011/4/12 0:00:00 |
THE GENETIC DIVERSITY OF THE 16S rRNA GENE IN THE MITOCHONDRIAL DNA OF THREE WILD POPULATIONS OF EXOPALAEMON CARINICAUDA |
| |
| MA Peng,LIU Ping,LI Jian,LI Ji-Tao and GAO Bao-Quan.THE GENETIC DIVERSITY OF THE 16S rRNA GENE IN THE MITOCHONDRIAL DNA OF THREE WILD POPULATIONS OF EXOPALAEMON CARINICAUDA[J].Oceanologia Et Limnologia Sinica,2012,43(1):174-179. |
| |
| Authors: | MA Peng LIU Ping LI Jian LI Ji-Tao and GAO Bao-Quan |
| |
| Institution: | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Ocean University of Dalian;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences |
| |
| Abstract: | Using PCR amplification and sequencing analysis, we examined the 16S rRNA gene in the mitochondrial gene of total 58 individuals that belonged to three wild populations of Exopalaemon carinicauda, which were sampled in the Haizhou, the Xiangshan, and the Laizhou Bays. 16S rRNA fragments that had 509bp were obtained in our study. Through comparing the variation sites, nucleotide diversity and average number of nucleotide differences were calculated to analyze the gene sequence variation and genetic diversity. The results showed that the colonies from the Haizhou and the Laizhou Bays had lower levels of genetic diversity than that from the Xiangshan Bay. Fst values between the Xiangshan and the Laizhou Bays showed significant genetic differences, and Fst values among all the other populations showed no strong genetic differences. The molecular phylogenetic tree was constructed using the NJ method to study molecular phylogeny of several species of Palaemonidae. Our finding indicated that the Phylogenetic tree clustered into two branches: Palaemoninae clustered into one branch, and Pontoniinae clustered into the other. In addition, differentiation time of 12 species of the Palaemonidae was deduced base on the percentage of the 16S rRNA divergence. |
| |
| Keywords: | Exopalaemon carinicauda 16S rRNA gene Genetics diversity Phylogenetic |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 |
|
