首页 | 本学科首页   官方微博 | 高级检索  
     检索      

厚壳贻贝(Mytilus coruscus)4 个群体遗传多样性的 ISSR 分析
引用本文:叶莹莹,徐梅英,郭宝英,吴常文.厚壳贻贝(Mytilus coruscus)4 个群体遗传多样性的 ISSR 分析[J].海洋与湖沼,2012,43(1):113-119.
作者姓名:叶莹莹  徐梅英  郭宝英  吴常文
作者单位:1. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心,舟山,316004
2. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心,舟山316004/浙江大海洋科技有限公司,舟山316004
3. 浙江海洋学院海洋科学学院国家海洋设施养殖工程技术研究中心,舟山316004/中国水产科学研究院东海水产研究所农业部海洋与河口渔业资源及生态重点开放实验室,上海200090
基金项目:国家科技部国际科技合作项目“浅海典型渔业生态系统功能恢复与重建关键技术”, 2009DFB20290 号; 国家海洋公益性行业科研专项, 201005013-05 号; 农业部海洋与河口渔业资源及生态重点开放实验室开放基金, 10-01 号; 2011 年浙江省大学生科技创新(新苗人才计划)项目, 2011.01—2012.06。
摘    要:采用ISSR分子标记对中国沿海4个厚壳贻贝群体进行遗传多样性和遗传结构分析,筛选出13条引物对4个群体120个样品共扩增出192个清晰的扩增位点,其中多态位点188个,多态位点百分率为97.92%,在物种水平上遗传多样性较为丰富。群体遗传多样性结果表明,PPL在81.77%—89.58%之间,H在0.2950—0.2818之间,I在0.4213—0.4447之间,其中福州群体多样性指数最低,温州群体最高。4个群体间GST为0.1519,Nm值范围介于3.0576—5.9714之间,AMOVA分析显示遗传变异主要来自群体内个体间。群体间遗传距离和聚类分析显示,温州群体和宁德群体首先聚为一支,再与舟山群体聚类,最后与福州群体聚在一起。表明地理位置较远的群体,遗传距离也较大,地理距离和遗传距离一致。

关 键 词:厚壳贻贝  群体遗传  遗传多样性  ISSR
收稿时间:2011/1/29 0:00:00
修稿时间:2011/3/26 0:00:00

ISSR ANALYSIS ON GENETIC STRUCTURE OF FOUR MYTILUS CORUSCUS POPULATIONS
YE Ying-Ying,XU Mei-Ying,GUO Bao-Ying and WU Chang-Wen.ISSR ANALYSIS ON GENETIC STRUCTURE OF FOUR MYTILUS CORUSCUS POPULATIONS[J].Oceanologia Et Limnologia Sinica,2012,43(1):113-119.
Authors:YE Ying-Ying  XU Mei-Ying  GUO Bao-Ying and WU Chang-Wen
Institution:Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Zhejiang Dahaiyang Science and Technology Co., Ltd.;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries R;Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture
Abstract:The genetic structure of four geographic populations of Mytilus coruscus along the coast of China was investigated by the inter-simple sequence repeat (ISSR) fingerprinting of 120 individual clams. Of the 192 ISSR loci tested, 188 (97.92%) were polymorphic with 13 ISSR primers. The genetic diversity was high at species level. The result showed that the PPL about four populations were among 81.77%—89.58%, H were among 0.2950—0.2818, I were among 0.4213—0.4447, respectively for the four populations with the lowest values in FZ population, the highest values in WZ population. The GST of the four populations was 0.1519, Nm was 3.0576—5.9714, AMOVA analysis showed that the genetic variation mainly from individuals within populations. Genetic distance and UPGMA tree showed that WZ was clustered with ND into one clad, then with ZS, and finally with FZ. The result showed that the further geographical distance, the higher genetic distance; the genetic distance is proportional to the geographical distance.
Keywords:Mytilus coruscus    Population genetics    Genetic diversity    ISSR
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《海洋与湖沼》浏览原始摘要信息
点击此处可从《海洋与湖沼》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号