| 青岛文昌鱼（Branchiostomabelcheri tsingtauense）染色体G类带型研究 |
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| 引用本文： | 牟洪善,王昌留,李金萍.青岛文昌鱼（Branchiostomabelcheri tsingtauense）染色体G类带型研究[J].海洋与湖沼,2011,42(4):597-602. |
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| 作者姓名： | 牟洪善 王昌留 李金萍 |
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| 作者单位： | 鲁东大学生命科学学院;东华理工大学学团部;鲁东大学生命科学学院;东华理工大学信息工程学院 |
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| 基金项目： | 国家“863”项目资助, 2008AA092604 号, 山东省教育厅项目资助, J08LE03 号, 江西省教育厅项目资助, GJJ11490 号 |
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| 摘 要： | 以青岛文昌鱼的晚期囊胚和早期原肠胚的胚胎细胞为实验材料，利用低渗处理、空气干燥法制备染色体玻片标本。在此基础上，为进一步探索显示青岛文昌鱼染色体G带的新方法，采用片龄、蛋白酶K和胰蛋白酶显带技术对青岛文昌鱼的染色体进行了初步研究，显示了青岛文昌鱼染色体的G类带型，绘制了青岛文昌鱼染色体的G类带型模式图，并对三种G类带型...
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| 关 键 词： | 青岛文昌鱼 染色体带型 G带 片龄 |
| 收稿时间： | 2010/3/15 0:00:00 |
| 修稿时间： | 2010/5/28 0:00:00 |
STUDIES ON THE G-TYPE CHROMOSOMAL PATTERNS OF AMPHIOXUS BRANCHIOSTOMA BELCHERI TSINGTAUENSE |
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| MU Hong-Shan,WANG Chang-Liu and LI Jin-Ping.STUDIES ON THE G-TYPE CHROMOSOMAL PATTERNS OF AMPHIOXUS BRANCHIOSTOMA BELCHERI TSINGTAUENSE[J].Oceanologia Et Limnologia Sinica,2011,42(4):597-602. |
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| Authors: | MU Hong-Shan WANG Chang-Liu and LI Jin-Ping |
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| Abstract: | The chromosomal patterns of amphioxus (Branchiostoma belcheri tsingtauens) were studied using blastula and early gastrula stages of embryos underwent hypotonic treatment and air-drying. New techniques for G-type banding were investigated using trypsin, protease K, and slide aging treatment. The G-type bandings of amphioxus were obtained and the chromosomal profiles were shown. The characteristics of the three G-type bands were compared, the similarities and differences were discussed. In addition, the correlation between the slide aging and G-banding was proposed. |
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| Keywords: | Amphioxus Branchiostoma belcheri tsingtauense Chromosome banding G-banding Slide aging |
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