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| 不同饥饿时段对黑鲷(Acanthopagrus schlegeli)ghrelin 基因表达的影响 | |
| 引用本文： | 马细兰,张 勇,刘 云,黄卫人,刘晓春,周立斌.不同饥饿时段对黑鲷(Acanthopagrus schlegeli)ghrelin 基因表达的影响[J].海洋与湖沼,2009,40(3):313-318. |
| 作者姓名： | 马细兰 张 勇 刘 云 黄卫人 刘晓春 周立斌 |
| 作者单位： | 1. 中山大学有害生物控制与资源利用国家重点实验室 水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室 广州 510275;惠州学院生命科学系 惠州 516007 2. 中山大学有害生物控制与资源利用国家重点实验室 水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室 广州 510275 3. 惠州学院生命科学系,惠州,516007 |
| 基金项目： | 国家"863计划"项目;2006AA10A402号，国家自然科学基金项目;30700611号，广东省自然科学基金项目;06027456号，广东省高校优秀青年创新人才培育项目;A2YM08094，惠州学院校立项目 |
| 摘 要： | 设计了黑鲷ghrelin基因的特异性引物，提取胃组织总RNA并扩增出目的片段，将PCR产物克隆到pGEM—TEasy载体，经质粒PCR扩增、酶切和测序鉴定重组质粒，构建标准曲线等，成功建立了ghrelin基因荧光实时定量PCR检测方法，以准确分析ghrelin基因在黑鲷摄食调节中的作用。运用建立的荧光实时定量PCR方法检测了不同饥饿时段（2天、7天）黑鲷胃、肠、下丘脑组织中ghrelin mRNA的表达变化。结果表明，饥饿2天（短期饥饿），黑鲷胃、肠组织中ghrelin mRNA表达显著上升（P〈0．05），下丘脑ghrelin mRNA表达无显著变化（P〉0．05）；饥饿7天（长期饥饿），黑鲷胃、肠组织ghrelin mRNA表达比饥饿2天显著下降，但仍显著高于对照组，下丘脑ghrelin mRNA表达逐渐升高（P〈0．05）；在同一饥饿时间内不同组织ghrelin mRNA表达存在显著差异（P〈0．05）。黑鲷不同组织ghrelin基因的表达随不同饥饿时段呈现出不同的变化趋势，推测ghrelin基因参与了黑鲷摄食调节的生物学讨程。 |
| 关 键 词： | 黑鲷 ghrelin 实时定量PCR 饥饿 |
| 收稿时间： | 2008/2/17 0:00:00 |
| 修稿时间： | 2008/4/18 0:00:00 |
EFFECT OF DIFFERENT FASTING STAGES ON GHRELIN EXPRESSION IN BLACK SEABREAM ACANTHOPAGRUS SCHLEGELI |
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| MA Xi-Lan,ZHANG Yong,LIU Yun,HUANG Wei-Ren,LIU Xiao-Chun and ZHOU Li-Bin.EFFECT OF DIFFERENT FASTING STAGES ON GHRELIN EXPRESSION IN BLACK SEABREAM ACANTHOPAGRUS SCHLEGELI[J].Oceanologia Et Limnologia Sinica,2009,40(3):313-318. | |
| Authors: | MA Xi-Lan ZHANG Yong LIU Yun HUANG Wei-Ren LIU Xiao-Chun and ZHOU Li-Bin |
| Institution: | State key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University; Department of Life Science, Huizhou University;State key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University;State key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University;State key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University;State key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University;Department of Life Science, Huizhou University |
| Abstract: | To analyze the roles of ghrelin in food intake of black seabream in detail, real-time quantitative PCR was adopted to examine the ghrelin gene expression. Total RNA was extracted from black seabream stomach tissue and the fragments of target gene were amplified by RT-PCR with special primers. PCR product was cloned via pGEM-T Easy vector and plasmid extracted. The recombinant plasmid was identified by PCR amplification and restriction enzyme analysis. Real time quantitative PCR was also applied to detect the ghrelin expression in stomach, intestine and hypothalamus tissues during different fasting stage. The results show that the expression of ghrelin increased in stomach and intestine tissue but not in hypothalamus tissue during a short period of fasting (2 days) (P<0.05) . However, during a long period of fasting (7 days), the expression decreased in stomach and intestine tissue, but still higher than that of the control (P<0.05), which was increased in hypothalamus tissue (P<0.05). Besides, the expression of ghrelin in different tissues at the same stage was different (P<0.05). The expression of ghrelin in different tissues did change in different fasting stages, suggesting that ghrelin is involved in the regulation of food intake in black seabream. |
| Keywords: | ghrelin |
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