| 瓦氏黄颡鱼(Pelteobagrus vachellii)脂肪酸去饱和酶FAD2和延伸酶ELOVL5的克隆及表达分析 |
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| 引用本文： | 覃川杰,文正勇,袁登越,邵婷,龚全.瓦氏黄颡鱼(Pelteobagrus vachellii)脂肪酸去饱和酶FAD2和延伸酶ELOVL5的克隆及表达分析[J].海洋与湖沼,2017,48(4):884-893. |
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| 作者姓名： | 覃川杰 文正勇 袁登越 邵婷 龚全 |
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| 作者单位： | 内江师范学院生命科学学院 长江上游鱼类资源保护与利用四川省重点实验室 内江 641112,内江师范学院生命科学学院 长江上游鱼类资源保护与利用四川省重点实验室 内江 641112,内江师范学院生命科学学院 长江上游鱼类资源保护与利用四川省重点实验室 内江 641112,内江师范学院生命科学学院 长江上游鱼类资源保护与利用四川省重点实验室 内江 641112,四川省农业科学院水产研究所 成都 611731 |
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| 基金项目： | 国家自然科学基金项目，31402305号；四川省科技厅应用基础项目，2017JY0161号；四川省“十三五”育种攻关项目，2016NYZ0024号。 |
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| 摘 要： | 脂肪酸去饱和酶(FAD,fatty acyl desaturase)及延伸酶(ELOVL,elongases of very long chain fatty acids)在鱼类脂肪代谢过程中发挥了重要作用。利用RT-PCR克隆得到瓦氏黄颡鱼(Pelteobagrus vachellii)肝脏中控制高不饱和脂肪酸合成的(FAD2)和(ELOVL5)基因cDNA序列。瓦氏黄颡鱼FAD2cDNA片段长2041bp,编码447个氨基酸,含有3个组氨酸簇(HDx GH,Hxx HH,Qxx HH),含亚铁血红素结合基序(HPGG)的类似细胞色素b5结构域等。瓦氏黄颡鱼ELOVL5 cDNA片段长1065bp,编码294个氨基酸,含有组氨酸簇(Hxx HH)、内质网停留信号(K、R)和4个ELO共有的保守区域(Kxx Exx DT,Qxx FLHx YHH,Nxxx Hxx MYx YY,Txx Qxx Q)等结构域。荧光定量PCR分析表明,FAD2和ELOVL5 m RNA在瓦氏黄颡鱼脑、肝脏的表达量最高,显著高于肠道、脾脏、肾脏、鳃等组织。结果表明,瓦氏黄颡鱼具有合成高不饱和脂肪酸的关键酶FAD2和ELOVL5,且肝脏为合成高不饱和脂肪酸的主要场所。
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| 关 键 词： | 脂肪酸去饱和酶(FAD2) 脂肪酸延伸酶(ELOVL5) 瓦氏黄颡鱼 cDNA |
| 收稿时间： | 2017/4/7 0:00:00 |
| 修稿时间： | 2017/4/25 0:00:00 |
MOLECULAR CLONING AND EXPRESSION OF FATTY ACID DESATURASE AND ELONGASE GENES IN DARKBARBEL CATFISH PELTEOBAGRUS VACHELLII |
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| QIN Chuan-Jie,WEN Zheng-Yong,YUAN Deng-Yue,SHAO Ting and GONG Quan.MOLECULAR CLONING AND EXPRESSION OF FATTY ACID DESATURASE AND ELONGASE GENES IN DARKBARBEL CATFISH PELTEOBAGRUS VACHELLII[J].Oceanologia Et Limnologia Sinica,2017,48(4):884-893. |
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| Authors: | QIN Chuan-Jie WEN Zheng-Yong YUAN Deng-Yue SHAO Ting and GONG Quan |
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| Institution: | Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China,Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China,Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China,Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China and Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, China |
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| Abstract: | Understanding the endogenous synthesis of long-chain polyunsaturated fatty acids is of benefit to fish feed formulation. A FADS2 and an ELOVL5 cDNA were cloned containing open reading frames (ORF) of 1338 base pair (bp) and 864 bp specifying proteins of 447 and 294 amino acids, respectively. The deduced amino acid sequence of Pelteobagrus vachellii FADS2 possessed conserved motif and included the histidine boxes, cytochrome b5 domain, transmembrane regions, and ELOVL 5 containing histidine boxes, endoplasmic reticulum retention signal domain, and transmembrane regions. In addition, FADS2 and ELOVL 5 mRNA distributed mainly in the brain and liver, and expressed significantly higher than that of intestine, spleen, kidney, gill, heart and muscle. These results indicate that P. vachellii possessed the desaturase and elongase, and the liver was the main tissues for synthesis of long-chain polyunsaturated fatty acids. |
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| Keywords: | fatty acid desaturase fatty acid elongase Pelteobagrus vachellii cDNA |
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