| 环境因子对南极菌Pseudoalteromonas sp.S-15-13多糖合成关键酶UGD基因表达的影响 |
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| 引用本文： | 李 江,沈继红,何培青,林学政,谭姣姣,李光友.环境因子对南极菌Pseudoalteromonas sp.S-15-13多糖合成关键酶UGD基因表达的影响[J].海洋与湖沼,2010,41(6):824-828. |
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| 作者姓名： | 李 江 沈继红 何培青 林学政 谭姣姣 李光友 |
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| 作者单位： | 1. 国家海洋局海洋生物活性物质重点实验室,青岛,266061;国家海洋局第一海洋研究所,青岛,266061
2. 青岛科技大学化工学院,青岛,266042 |
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| 基金项目： | 国家自然科学基金, 40706053 号; 国家 863 科技计划重点项目, 2007AA091905 号 |
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| 摘 要： | 以南极菌Pseudoalteromonas sp.S-15-13为材料,采用实时定量PCR的方法研究了温度、冻融循环及培养基中NaCl、葡萄糖含量和pH对多糖合成关键酶基因ugd表达水平的影响。结果表明,低温有利于ugd的表达,在2℃和10℃培养温度下,培养24h后ugd的表达量约为20℃时的4倍;冻融循环后,ugd的表达量上调,第2个冻融循环后ugd的表达量较对照提高了6.85倍;NaCl对ugd的影响表现为低促高抑,即NaCl含量为6.0%时ugd的表达量是对照(3.0%)的3.97倍,但含量达9.0%时ugd表达量仅为对照的2/5;葡萄糖能够提高ugd的表达量,当含量为2.0%时ugd表达量为对照的13.68倍;在一定范围内(pH5.0—8.0),pH的改变会促进ugd的表达,当pH为6.0时ugd表达量约为pH7.0时的2.15倍。
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| 关 键 词： | 南极菌 Pseudoalteromonas sp.S-15-13 Real-time PCR UGD 环境因子 |
| 收稿时间： | 2009/12/15 0:00:00 |
EFFECTS OF ENVIRONMENTAL FACTORS ON THE UGD GENE EXPRESSION OF ANTARCTIC BACTERIUM PSEUDOALTEROMONAS SP. S-15-13 |
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| LI Jiang,SHEN Ji-Hong,HE Pei-Qing,LIN Xue-Zheng,TAN Jiao-Jiao and LI Guang-You.EFFECTS OF ENVIRONMENTAL FACTORS ON THE UGD GENE EXPRESSION OF ANTARCTIC BACTERIUM PSEUDOALTEROMONAS SP. S-15-13[J].Oceanologia Et Limnologia Sinica,2010,41(6):824-828. |
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| Authors: | LI Jiang SHEN Ji-Hong HE Pei-Qing LIN Xue-Zheng TAN Jiao-Jiao and LI Guang-You |
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| Institution: | Key Laboratory of Marine Bio-active Substances, State Oceanic Administration;First Institute of Oceanorgarphy, State Oceanic Administration;Key Laboratory of Marine Bio-active Substances, State Oceanic Administration;First Institute of Oceanorgarphy, State Oceanic Administration;Key Laboratory of Marine Bio-active Substances, State Oceanic Administration;First Institute of Oceanorgarphy, State Oceanic Administration;Key Laboratory of Marine Bio-active Substances, State Oceanic Administration;First Institute of Oceanorgarphy, State Oceanic Administration;College of Chemical Engineering, Qingdao University of Science & Technology;Key Laboratory of Marine Bio-active Substances, State Oceanic Administration;First Institute of Oceanorgarphy, State Oceanic Administration |
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| Abstract: | |
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| Keywords: | Antarctic bacterium Pseudoaltermonas sp S-15-13 Real-time PCR UGD Environmental factors |
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