| 圆紫菜(Pyropia suborbiculata)减数分裂时期的遗传分析与叶状体形态建成 |
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| 引用本文： | 杨秀文,丁洪昌,严兴洪,刘长军.圆紫菜(Pyropia suborbiculata)减数分裂时期的遗传分析与叶状体形态建成[J].海洋与湖沼,2019,50(1):173-180. |
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| 作者姓名： | 杨秀文 丁洪昌 严兴洪 刘长军 |
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| 作者单位： | 水产种质资源发掘与利用教育部重点实验室(上海海洋大学) 上海201306;水产科学国家级实验教学示范中心(上海海洋大学) 上海201306;上海水产养殖工程技术研究中心(上海海洋大学) 上海201306;象山县水产技术推广站 象山315700 |
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| 基金项目： | 国家重点研发计划“蓝色粮仓科技创新”重点专项，2018YFD0900606号；国家自然科学基金资助项目，31072208号；浙江省农业（水产）新品种选育重大科技专项，2016C02055-6号；江苏省科技计划（现代农业）重点项目，BE2018335号。 |
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| 摘 要： | 本文以圆紫菜的绿色突变体(LT)和红色突变体(HT)进行杂交实验,证实了圆紫菜减数分裂发生的时期,并观察了圆紫菜的早期发育和形态建成过程。杂交F1叶状体中,出现了2种亲本色和2种重组色,它们分别为绿色(G,母本色)、红色(R,父本色)、野生色(W)和黄褐色(Y)。4种颜色在F1叶状体中形成了大量由2—4个色块组成的颜色嵌合体,色块出现了分离并呈线性排列。F1叶状体中嵌合体所占比例为82.9%,其中两嵌合最多,三嵌合次之,四嵌合最少。4种颜色在嵌合体的分离比为1G:0.97R:0.88W:0.69Y。上述结果证实圆紫菜的减数分裂发生在壳孢子萌发初期。减数分裂产生的四分子呈线形排列,随后继续发育成叶状体,叶状体发育至7—9个细胞时进行第一次纵向分裂,叶状体形态变宽。随后,圆紫菜梢部开始大量形成和放散单孢子,最终导致圆紫菜的形状为圆形或肾脏形。
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| 关 键 词： | 圆紫菜 色素突变体 杂交实验 减数分裂 色块嵌合体 形态建成 |
| 收稿时间： | 2018/3/30 0:00:00 |
| 修稿时间： | 2018/7/1 0:00:00 |
GENETIC ANALYSIS ON THE PERIOD OF MEIOSIS AND MORPHOGENESIS IN GAMETOPHYTIC BLADES OF PYROPIA SUBORBICULATA (BANGIALES, RHODOPHYTA) |
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| YANG Xiu-Wen,DING Hong-Chang,YAN Xing-Hong and LIU Chang-Jun.GENETIC ANALYSIS ON THE PERIOD OF MEIOSIS AND MORPHOGENESIS IN GAMETOPHYTIC BLADES OF PYROPIA SUBORBICULATA (BANGIALES, RHODOPHYTA)[J].Oceanologia Et Limnologia Sinica,2019,50(1):173-180. |
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| Authors: | YANG Xiu-Wen DING Hong-Chang YAN Xing-Hong and LIU Chang-Jun |
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| Institution: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China and Xiangshan Fisheries Technical Extension Center, Xiangshan 315700, China |
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| Abstract: | A cross experiment was carried out for two artificial pigment mutants to study the position of meiosis in P. suborbiculata. The early development and morphogenesis of P. suborbiculata blades were observed. Four types of unsectored blades and 84 types sectored blades in F1 blades were found. Except for the two parents color types blades, a wild and a yellow-brown type blades were discovered. The sectored F1 blades represented 82.9% of the total blades, and the chimera was composed mainly of two-sectored blades and three-sectored blades, accounting for 43.4% and 32.7%, respectively. Sectors of these four colors appeared in the sectored F1 blades in the proportion of 870G:840R:766W:602Y, being approximately 1:0.95:0.88:0.69. The results indicate that the meiosis of P. suborbiculata occurs during the first two cell divisions of the germinating conchospores. The linear four cells that resulted from the meiosis continued to develop, and the first longitudinal division began at the blades division into 7-9 cells. Afterwards, a large number of monospores formed and were released in the top of the blade, which eventually led to round or kidney-shaped morphology of P. suborbiculata. |
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| Keywords: | Pyropia suborbiculata pigmentation mutant cross experiment meiosis color chimera thallus morphogenesis |
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