| 小球藻对5种常用基因工程抗生素的敏感性研究 |
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| 引用本文： | 陈颖,李文彬,张利明,孙勇如.小球藻对5种常用基因工程抗生素的敏感性研究[J].海洋与湖沼,1999,30(5):500-505. |
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| 作者姓名： | 陈颖 李文彬 张利明 孙勇如 |
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| 作者单位： | 中国科学院遗传研究所!北京，100101，中国科学院遗传研究所!北京，100101，中国科学院遗传研究所!北京，100101，中国科学院遗传研究所!北京，100101 |
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| 基金项目： | 国家“九五”科技攻关资助!96-C02-04-05 |
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| 摘 要： | 于１９９６年以本实验室培养的随圆小球藻为材料，就其对Ｇ４１８，潮霉素，氯霉素，链霉素，卡好霉素等５种抗生素的敏感性进行了研究。结果表明，小球藻对氯霉素，链霉素和卡那霉素敏感，对潮霉素较为敏感，１００μｇ／ｍｌ即０．１９０ｍｍｏｌ／Ｌ的用量可抑制固体培养中小球藻的生长；对Ｇ４１８则高度敏感，３０μｇ／ｍｌ即０．０４３ｍｍｏｌ／Ｌ的Ｇ４１８即可完全抑制固体培养中小藻 的生长，１５μｇ／ｍｌ即０．０２２
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| 关 键 词： | 小球藻 基因工程 选择试剂 抗生素 敏感性 |
| 收稿时间： | 1/8/1998 12:00:00 AM |
| 修稿时间： | 1998/6/29 0:00:00 |
STUDY ON SENSITIVITIES OF CHLORELLA ELLIPSOIDEA TO 5 ANTIBIOTICS IN GENETIC ENGINEERING |
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| CHEN Ying,LI Wen-bin,Zhang Li-ming and SUN Yong-ru.STUDY ON SENSITIVITIES OF CHLORELLA ELLIPSOIDEA TO 5 ANTIBIOTICS IN GENETIC ENGINEERING[J].Oceanologia Et Limnologia Sinica,1999,30(5):500-505. |
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| Authors: | CHEN Ying LI Wen-bin Zhang Li-ming and SUN Yong-ru |
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| Institution: | Institute of Genetics, me Chinese Academy of Sciences, Beijing, 100101;Institute of Genetics, me Chinese Academy of Sciences, Beijing, 100101;Institute of Genetics, me Chinese Academy of Sciences, Beijing, 100101;Institute of Genetics, me Chinese Academy of Sciences, Beijing, 100101 |
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| Abstract: | Much progress has been made for recent years in selection markers of high plants.However, the situation varies for different algae. Researches on Chlamdomynas reinhardtii developedrapidly and there is also a good start in macroalgae, such as Laminaria japonica, but little progresshas been made for Chlorella. Lack of selection marker made the use of gene transferred Chlorellalimited. Ths paper reports a study on sensitivities of Chlorella ellipsoidea to 5 antibiotics in orderto identify suitable selechon marker of Chlorella genetic engineering.Chlorella ellipsoidea cells after 4-5 days' culture were used as material and antibiotics-Hygromycin (Hm), Chloromycehn (Cm), and Streptomycin (Str) and Kanamycin (Km), differentconcentrahons were used for test in solid culture and Genehcin (G418) was used in solid and liquidculture. After 1-5 weeks' culture, the results show that C. ellipsoidea is not sensitive to Cm, Strand Km, 600ug / ml or 1.240mmol / L Km, 600ug / ml or 0.412mmol / L Str and 200ug / ml or0.169mmol/ L Cm cannot inhabit the growth of C. ellipsoidea. However, it is relatively sensitive toHm, 100ug / ml or 0.191mmol / L Hm can completely stop the growth of C. ellipsoidea on solidmedia. Further, C. ellipsoidea was highly sensitive to G418, since 30ug / ml or 0.043mmol / L and15ug / ml or 0.022mmol / L completely inhabited the growth of C. ellipsoidea in solid and liquidcultures, respectively. It suggests that G418 be the most suitable selection regent if using NPTII geneas selection marker for C. ellipsoidea genetic engineering. |
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| Keywords: | Chlorella ellipsoidea Genetic engineering Selection marker |
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