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山东近海褐牙鲆自然与养殖群体生化遗传结构及其遗传变异的比较分析
引用本文:尤锋,王可玲,相建海,徐成.山东近海褐牙鲆自然与养殖群体生化遗传结构及其遗传变异的比较分析[J].海洋与湖沼,2001,32(5):512-518.
作者姓名:尤锋  王可玲  相建海  徐成
作者单位:中国科学院海洋研究所
基金项目:国家重点基础研究项目G1 9990 1 2 0 0 0课题资助,国家科委攀登计划B资助项目 PDB6-5 -2号
摘    要:山东近海褐牙鲆自然群体活样本共 79尾 ,分别于 1 996年 5月、1 997年 1月和 1 998年 4月采自青岛近海 ;养殖群体活样本 5 2尾于 1 997年 1 2月采自山东荣成寻山养鱼场。采用水平淀粉胶和垂直聚丙烯酰胺凝胶电泳方法及 3种缓冲系统 (TC、EBT和TG)分别对自然和养殖群体的 1 5种同工酶进行生化遗传分析。结果表明 ,山东近海牙鲆自然群体的多态基因座位比例 ( 31 .0 % )和群体平均杂合度 ( 0 .0 80 2 )都明显高于养殖群体 ( 2 4 .1 % ,0 .0 788) ;在自然群体的 9个多态基因座位、养殖群体的 7个多态基因座位中 ,除了Cat(P <0 .0 5 )和Idhp - 1(P <0 .0 5 ,养殖群体中 )有显著差异、Ldh -C(P <0 .0 1 )完全偏离Hardy -Weinberg定律外 ,其余多态座位基因频率均符合Hardy-Weinberg遗传平衡定律

关 键 词:褐牙Ping  同工酶  生化遗传结构  养殖群体  遗传变异  山东  自然群体
收稿时间:2000/6/15 0:00:00
修稿时间:2000年6月15日

COMPARATIVE ANALYSIS OF BIOCHEMICAL GENETIC STRUCTURE AND VARIANCE BETWEEN NATURAL AND CULTURED STOCKS ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.& S.) OFF SHANDONG COASTAL WATERS
YOU Feng,WANG Ke-Ling,XIANG Jian-Hai and XU Cheng.COMPARATIVE ANALYSIS OF BIOCHEMICAL GENETIC STRUCTURE AND VARIANCE BETWEEN NATURAL AND CULTURED STOCKS ON THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T.& S.) OFF SHANDONG COASTAL WATERS[J].Oceanologia Et Limnologia Sinica,2001,32(5):512-518.
Authors:YOU Feng  WANG Ke-Ling  XIANG Jian-Hai and XU Cheng
Institution:Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071;Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071;Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071;Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071
Abstract:Expressions of 15 isozymes (LDH, MDH, MEP, IDHP, G3PDH, AK, CK, ACP, PGM, SDH, ADH, CAT, SOD, GDH and GPI) on the left-eyed flounder, Paralichthys olivaceus, including natural and cultured stocks off Shandong coastal waters of China, were tested by horizontal starch gel and vertical polyacrylamidae gel electrophoresis in 3 buffer systems (TC, pH= 6.9; EBT, pH= 8.9; TG, pH= 8.3. The natural sample size is 79 live left-eyed flounders, collected from the coastal waters of Qingdao from May in 1996 to April in 1998. The cultured samples (52 live left-eyed flounders) were collected from a fish farm in Rongcheng of Shandong Province in December, 1997, the parental generation of which was also captured in the coastal waters of Shandong. Four tissues and organs i.e. eye, muscle, heart and liver were fetched from each of the samples after regular morphological measurement and tested at once or stored in liquid nitrogen (-196°C) for test. The biochemical genetic structures of both stocks have been studied and compared. Altogether 29 loci in each stock were recorded, among which 9 gene loci (Ldh-A, Ldh-C, Idhp-1, Acp-1, Pgm, Sdh, Adh, Cat and Gdh) belong to the polymorphic loci in the natural stock and its mean proportion of polymorphic loci (P) was 31.0%. In the cultured stock, 7 gene loci (Ldh-A, Ldh-C, Idhp-1, Sdh, Adh, Cat and Gdh) belong to the polymorphic loci and its P value was 24.1%. The allelic frequencies of both stock were also gained, with the average heterozygosities (H) of stock being 0.0802 in natural stock and 0.0788 in cultured stock. The values of P and H in the cultured stock were all lower than those in the natural stock; hence the way to keep the quality of the cultured filial generation and then to increase fish production are very important for fish farming. The chi-square test shows that polymorphic loci but Cat (P<0.05), Idhp-1 (P<0.05), only in the cultured population) and Ldh-C (P<0.01) in both the natural and cultured stock were in Hardy-Weinberg equilibrium. In the mean time, the values of observed heterozygosity (Ho), Hardy-Weinberg expected heterozygosity (He) and genetic deviate index (d) also expressed the difference in genetic variance of these two stocks.
Keywords:Paralichthys olivaceus  Isozyme  Biochemica l genetic structure  Natural and cultured stocks  Genetic variance
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