| 鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)磷酸酶活性的影响 |
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| 引用本文： | 宋 欣,张丽岩,高玮玮,潘宝平.鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)磷酸酶活性的影响[J].海洋与湖沼,2010,41(2):254-258. |
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| 作者姓名： | 宋 欣 张丽岩 高玮玮 潘宝平 |
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| 作者单位： | 天津师范大学化学与生命科学学院,细胞遗传与分子调控天津市重点实验室,天津,300387 |
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| 基金项目： | 天津市科委应用基础与前沿技术重点项目资助,09JCZDJC19300号;;天津市科委应用基础研究面上项目资助,06YFJMJC11800号 |
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| 摘 要： | 对500个成体青蛤分别用鳗弧菌和生理盐水注射,在感染后3h、6h、12h、24h和36h分别取不同处理组的肝胰脏、鳃和闭壳肌组织,测定上述样品的碱性磷酸酶(ALP)及酸性磷酸酶(ACP)活性,分析鳗弧菌对青蛤体内免疫相关酶活性的影响。结果表明,鳗弧菌感染组的ALP与ACP活性从高到低依次为肝胰脏鳃闭壳肌,其中青蛤肝胰脏和鳃组织中ALP和ACP活性均有显著升高的趋势,并且在12h时达到最高,与对照组差异显著(P0.05),随后呈现下降趋势。而青蛤闭壳肌组织中侵染组ALP和ACP活性与对照组之间没有显著差异(P0.05)。鳗弧菌对青蛤的磷酸酶活性影响较大,对其免疫防御系统有明显的刺激作用。
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| 关 键 词： | 青蛤 鳗弧菌 碱性磷酸酶（ALP） 酸性磷酸酶（ACP） |
| 收稿时间： | 2009/5/17 0:00:00 |
| 修稿时间： | 2009/7/18 0:00:00 |
EFFECT OF VIBRIO ANGUILLARUM ON ACTIVITY OF PHOSPHATASE IN CYCLINA SINENSIS |
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| SONG Xin,ZHANG Li-Yan,GAO Wei-Wei and PAN Bao-Ping.EFFECT OF VIBRIO ANGUILLARUM ON ACTIVITY OF PHOSPHATASE IN CYCLINA SINENSIS[J].Oceanologia Et Limnologia Sinica,2010,41(2):254-258. |
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| Authors: | SONG Xin ZHANG Li-Yan GAO Wei-Wei and PAN Bao-Ping |
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| Institution: | College of Chemistry and Life Science;Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation;Tianjin Normal University;Tianjin;300387 |
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| Abstract: | In this study, Vibrio anguillarum and normal saline were injected separately into 500 adult clams (Cyclina sinensis). At 3h, 6h, 12h, 24h, and 36h after injection, hepatopancreas, gills, and adductor muscle tissues of the samples in different treatment groups were collected, and alkaline phosphatase (ALP) and acid phosphatase (ACP) activities were measured in order to reveal the relationship between V. anguillarum injection and immune-related response. The results show that after the injection, ALP and ACP ... |
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| Keywords: | Cyclina sinensis Vibrio anguillarum Alkaline phosphatase (ALP) Acid phosphatase (ACP) |
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