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| 中国沿海长蛸(Octopus variabilis)自然群体线粒体COI基因遗传多样性研究 | |
| 引用本文： | 孙宝超,杨建敏,孙国华,刘相全,刘丽娟,王卫军,郑小东.中国沿海长蛸(Octopus variabilis)自然群体线粒体COI基因遗传多样性研究[J].海洋与湖沼,2010,41(2):259-265. |
| 作者姓名： | 孙宝超 杨建敏 孙国华 刘相全 刘丽娟 王卫军 郑小东 |
| 作者单位： | 1. 山东省海洋水产研究所,烟台,264006;上海海洋大学,上海,201306 2. 山东省海洋水产研究所,烟台,264006 3. 中国海洋大学,青岛,266003 |
| 基金项目： | 山东省优秀中青年科学家奖励基金(博士基金),2006BS06010号;;国家“863”计划“中国近海贝类资源的采集、保存与信息化技术”,2007AA09Z433号 |
| 摘 要： | 对我国沿海5个自然群体长蛸的线粒体细胞色素氧化酶亚基I(COI)部分序列进行了测定和分析,经比对获得658bp核苷酸片段,其中碱基T、C、A和G的平均含量分别为37.02%、18.46%、30.15%和14.35%,AT的含量明显高于GC的含量。5个自然群体的长蛸中共发现18个变异位点,得到15个单倍型,包括4个共享单倍型,其中青岛群体的核苷酸差异数K以及平均核苷酸多样性指数Pi最高,烟台群体最低;群体间,青岛群体在群体间核苷酸差异数K、群体间平均每位点核苷酸替代数Dxy和群体间每位点净核苷酸替代数Da三个指标上都表现出比其它群体之间较高的水平,说明青岛群体具有最为丰富的遗传多样性。MEGA3.1软件计算5个群体间的Kimura2-paramter遗传距离,大连群体和青岛群体之间的遗传距离最远为0.0077,而大连群体与烟台群体之间的遗传距离最低,为0.0029。AMOVA分析表明,五群体间总遗传分化系数Fst=0.17410(P0.05),群体间遗传分化远小于群体内。NJ法和UPGMA法构建的分子进化树,5个地理群体的长蛸聚为两个族群,大连、烟台群体聚为一族群,青岛、连云港和舟山群体聚为另一族群。 |
| 关 键 词： | 长蛸 COI基因 PCR 遗传多样性 |
| 收稿时间： | 2009/5/15 0:00:00 |
| 修稿时间： | 2009/7/12 0:00:00 |
SEQUENCE AND MOLECULAR PHYLOGENY OF MITOCHONDRIAL COI GENE FRAGMENT IN FIVE POPULATIONS OF OCTOPUS VARIABILIS IN CHINA |
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| SUN Bao-Chao,YANG Jian-Min,SUN Guo-Hu,LIU Xiang-Quan,LIU Li-Juan,WANG Wei-Jun and ZHENG Xiao-Dong.SEQUENCE AND MOLECULAR PHYLOGENY OF MITOCHONDRIAL COI GENE FRAGMENT IN FIVE POPULATIONS OF OCTOPUS VARIABILIS IN CHINA[J].Oceanologia Et Limnologia Sinica,2010,41(2):259-265. | |
| Authors: | SUN Bao-Chao YANG Jian-Min SUN Guo-Hu LIU Xiang-Quan LIU Li-Juan WANG Wei-Jun and ZHENG Xiao-Dong |
| Institution: | 1. Shandong Marine Fisheries Research Institute;Yantai;264006;2. Shanghai Ocean University;Shanghai;201306;3. Ocean University of China;Qingdao;266003 |
| Abstract: | Mitochondrial COI gene fragments of wild Octopus variabilis were amplified with PCR; the PCR products were purified and sequenced, and 658bp nucleotide sequences were obtained. The T, C, A, and G contents in this fragment were 37.02%, 18.46%, 30.15%, and 14.35%, respectively. The AT content was higher than the GC content. 18 variable nucleotide positions were detected in 658bp COI sequences analyzed; 15 haplotypes are defined among five populations, of which only 4 were common. Among the five populations, Q... |
| Keywords: | Octopus variabilis COI gene PCR Genetic diversity |
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