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栉孔扇贝16S rRNA基因片段序列的多态性研究
引用本文:刘亚军,喻子牛,姜艳艳,张留所,孔晓瑜,宋林生.栉孔扇贝16S rRNA基因片段序列的多态性研究[J].海洋与湖沼,2002,33(5):477-483.
作者姓名:刘亚军  喻子牛  姜艳艳  张留所  孔晓瑜  宋林生
作者单位:1. 青岛海洋大学教育部海水养殖重点实验室,青岛,266003
2. 中国科学院海洋研究所,青岛,266071
基金项目:国家重点基础研究发展规划项目 (973 )资助G1 9990 1 2 0 0 8号,国家自然科学基金资助项目 3 960 0 1 1 3号
摘    要:采用PCR技术扩增了栉孔扇贝(Chlamys farreri)线粒体DNA的16SrRNA基因片段,PCR产物经T载体连接之后进行了克隆,测序,得到634bp的核苷酸序列;分析了该片段的大连,烟台,青岛,朝鲜4个自然群体31个个体的核苷酸序列多态性,共检测到26个多态性核苷酸位点,18种单倍型,结果表明,4个群体中以朝鲜群体遗传多样性最高,其次为烟台,青岛群体,大连群体最低;不同自然分布区的栉孔扇贝未出现明显的遗传分化。

关 键 词:栉孔扇贝  线粒体DNA  16SrRNA基因  DNA序列测定  PCR技术  克隆  核苷酸序列  多态性
收稿时间:2001/11/6 0:00:00
修稿时间:2001年11月6日

SEQUENCE POLYMORPHISM OF MITOCHONDRIAL 16S rRNA GENE FRAGMENT IN SCALLOP CHLAMYS FARRERI
LIU Ya-Jun,YU Zi-Niu,JIANG Yan-Yan,ZHANG Liu-Suo,KONG Xiao-Yu and SONG Lin-Sheng.SEQUENCE POLYMORPHISM OF MITOCHONDRIAL 16S rRNA GENE FRAGMENT IN SCALLOP CHLAMYS FARRERI[J].Oceanologia Et Limnologia Sinica,2002,33(5):477-483.
Authors:LIU Ya-Jun  YU Zi-Niu  JIANG Yan-Yan  ZHANG Liu-Suo  KONG Xiao-Yu and SONG Lin-Sheng
Institution:Key Laboratory of Mariculture, Ministry of Education , Ocean University of Qingdao;Key Laboratory of Mariculture, Ministry of Education , Ocean University of Qingdao;Key Laboratory of Mariculture, Ministry of Education , Ocean University of Qingdao;Key Laboratory of Mariculture, Ministry of Education , Ocean University of Qingdao;Key Laboratory of Mariculture, Ministry of Education , Ocean University of Qingdao;Institute of Oceanology , The Chinese Academy of Sciences
Abstract:DNA sequencing is very useful for the genetic diversity analysis at molecular level. Mitochondrial 16S rRNA gene fragment of Scallop Chlamys farreri was amplified via polymerase chain reaction (PCR), the PCR products were ligated into T vector, cloned and sequenced for 31 individuals, 3 populations of China (8 for Dalian and Qingdao each, and 7 for Yantai) and one population of Republic of Korea. 634 base pair nucleotide sequences were examined and analyzed for genetic polymorphism. Resultant sequences data analysis showed that all 31 sequences were grouped into 18 haplotypes and there were 26 variable nucleotide positions in this gene fragment, which included transitions and transvertions, and no insertion/deletion were observed. The genetic diversity indexes including S (polymorphic sites), P (nucleotide diversity) and K (average number of nucleotide difference) were calculated and the results indicated that Korea population showed more variation than others and more differences to others, followed by Yantai and Qigndao population, and Dalian population had the least sequence variation. Parameters of genetic variation among the four populations suggested that no significant genetic difference was observed. Our data provided baseline information at DNA level on the genetic variation in wild Scallop populations and may be useful for future management and utilization of the resource.
Keywords:Chlamys farreri      Mitochondrial DNA (mtDNA)    16S rRNA gene    DNA sequencing
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