| 大黄鱼(Larimichthys crocea)补体C3和C4基因的分子特征及表达分析 |
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| 引用本文： | 王海玲,祁鹏志,郭宝英,吴常文.大黄鱼(Larimichthys crocea)补体C3和C4基因的分子特征及表达分析[J].海洋与湖沼,2015,46(1):181-190. |
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| 作者姓名： | 王海玲 祁鹏志 郭宝英 吴常文 |
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| 作者单位： | 浙江海洋学院 国家海洋设施养殖工程技术研究中心 舟山 316022 |
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| 基金项目： | 国家自然科学基金项目,31272643号,31302215号;山东省自然科学基金项目,ZR2013CQ030号;烟台市科技发展计划项目,2011068号;水生动物营养与饲料"泰山学者"岗位资助。 |
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| 摘 要： | 本文测定了大黄鱼C3(L.c-C3)和C4(L.c-C4)基因的c DNA全序列。结果表明,L.c-C3和L.cC4序列全长分别为4962bp和5088bp,分别编码1653和1695个氨基酸,N端信号肽序列分别为23和19个氨基酸。推导的氨基酸序列结构分析表明大黄鱼C3和C4与已报道的补体C3、C4同样都具有在功能上比较重要的残基以及保守的硫酯区。分子进化分析表明,L.c-C3和L.c-C4分别与鮸鱼C3、C4的氨基酸同源性最高。实时荧光定量PCR结果显示,L.c-C3和L.c-C4在健康大黄鱼的肝脏、脾脏、肠、鳃、心脏、脑、肌肉和胃这8种组织中都有表达,其中肝脏的表达量最高。在大黄鱼胚胎不同发育时期(从2细胞期到初生仔鱼)中,L.c-C3在各个阶段没有明显的变化,而L.c-C4的表达量有明显升高。溶藻弧菌(Vibrio alginolyticus)侵染的大黄鱼肝脏和脾脏中,L.c-C3和L.c-C4的m RNA表达量均明显上调。该结果表明,大黄鱼肝组织C3和C4基因表达变化与溶藻弧菌的侵染密切相关,揭示了C3和C4在大黄鱼抗细菌免疫反应中具有重要的作用。
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| 关 键 词： | 大黄鱼 大黄鱼补体C3(L.c-C3) 大黄鱼补体C4(L.c-C4)序列特点 分子特征 表达分析 |
| 收稿时间： | 2014/10/23 0:00:00 |
| 修稿时间： | 2014/11/28 0:00:00 |
MOLECULAR CHARACTERIZATION AND EXPRESSION OF COMPLEMENT COMPONENT C3 AND C4 IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA |
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| WANG Hai-Ling,QI Peng-Zhi,GUO Bao-Ying and WU Chang-Wen.MOLECULAR CHARACTERIZATION AND EXPRESSION OF COMPLEMENT COMPONENT C3 AND C4 IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA[J].Oceanologia Et Limnologia Sinica,2015,46(1):181-190. |
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| Authors: | WANG Hai-Ling QI Peng-Zhi GUO Bao-Ying and WU Chang-Wen |
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| Institution: | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China |
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| Abstract: | A full-length cDNA encoding OoBtk was cloned from the cDNA Library of Octopus ocellatus in this study. It was 1191 bp, containing a 5' untranslated region of 259 bp, a 3'UTR of 277 bp, and an open reading frame (ORF) of 705 bp encoding a polypeptide of 234 amino acids with an isoelectric point of 8.50 and predicted molecular weight of 27.7 kDa. The mRNA expression patterns of an OoBtk in tissues and haemocytes after Listonella anguillarum or Micrococcus luteus challenge were characterized by real-time PCR. The results show that OoBtk was widely expressed in muscle, mantle, gill, branchial heart, systemic heart, hepatopancreas, saccus renalis, stomach, gonad, and hemocyte, highest in hepatopancreas. The mRNA expression of OoBtk in hemocytes showed an obviously induced trend after L. anguillarum or M. luteus stimulation; it was significantly up-regulated at 6h post stimulation, and peaked at 48h or 12h post stimulation, respectively. Therefore, OoBtk in O. ocellatus is involved in the response against L. anguillarum or M. luteus challenge. |
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| Keywords: | Octopus ocellatus tyrosine protein kinase real-time PCR Listonella anguillarum Micrococcus luteus |
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