| 去污剂对裙带菜色素-蛋白质复合物电泳分离效果及其性质的影响 |
| |
| 引用本文： | 李爱芬,陈敏,周百成.去污剂对裙带菜色素-蛋白质复合物电泳分离效果及其性质的影响[J].海洋与湖沼,2001,32(4):394-401. |
| |
| 作者姓名： | 李爱芬 陈敏 周百成 |
| |
| 作者单位： | 中国科学院海洋研究所 |
| |
| 基金项目： | 国家“九五”攻关资助项目96-C0 1 -0 5-0 1号 |
| |
| 摘 要： | 于1998年3月在青岛市太平角采集裙带菜孢子体，通过破碎、离心制备类囊体膜，用去污剂SDS、DOC、DMG和DIG增溶裙带菜类囊体膜，经PAGE分离色素-蛋白质复合物，并测定其光谱特性，结果表明，电泳分离效果与所用的去污剂的种类有关。去污剂不同，分离到的同一类型复合物的吸收光谱有差异，捕光色素-蛋白质复合物的数目、种类及吸收光谱明显不同，电泳中产生的游离色素的多少也随去污剂种类而异，在4种去污剂中，以光谱明显不同，电泳中产生的游离色素的多少也随去污剂种类而异，在4种去污剂中，以DMG和DIG增溶的效果最好，经SDS-PAGE分出6种色素-蛋白质复合物，游离色素较少，DMG和DIG增溶的效果最好，经SDS-PAGE分出6种色素-蛋白质复合物，游离色素较少。
|
| 关 键 词： | 裙带菜 去污剂 凝胶电泳 色素－蛋白质复合物 吸收光谱 荧光光谱 分离效果 |
| 收稿时间： | 1999/6/22 0:00:00 |
| 修稿时间： | 1999年6月22日 |
EFFECT OF DETERGENTS ON ELECTROPHORETIC SEPARATION AND PROPERTIES OF PIGMENT-PROTEIN COMPLEXES FROM A BROWN ALGA UNDARIA PINNATIFIDA |
| |
| LI Ai-Fen,CHEN Min and ZHOU Bai-Cheng.EFFECT OF DETERGENTS ON ELECTROPHORETIC SEPARATION AND PROPERTIES OF PIGMENT-PROTEIN COMPLEXES FROM A BROWN ALGA UNDARIA PINNATIFIDA[J].Oceanologia Et Limnologia Sinica,2001,32(4):394-401. |
| |
| Authors: | LI Ai-Fen CHEN Min and ZHOU Bai-Cheng |
| |
| Institution: | Institute of Oceanology,The Chinese Academy of Sciences,Qingdao 266071;Institute of Oceanology,The Chinese Academy of Sciences,Qingdao 266071;Institute of Oceanology,The Chinese Academy of Sciences,Qingdao 266071 |
| |
| Abstract: | The thylakiod membranes isolated from a brown alga Undaria pinnatifida were solubilized by detergents sodium dodecyl sulfate (SDS), deoxycholate (DOC), decanoyl-N-methylglucamide (DMG) and digitonin (DIG) at different concentrations (SDS:Chl=10:1, DOC:Chl=60:1, DMG:Chl=20:1, DIG:Chl =50:1). The pigment-protein complexes were separated by the SDS-PAGE, and their spectrum characteristics were determined. The results of PAGE separation varied with detergents. DOC solubilization yielded 10 pigment-containing bands and resulted in a large number of free pigments. SDS solubilization yielded only 5 pigment-containing bands and lots of free pigment were produced. Comparing with SDS and DOC, DMG and DIG solubilization resulted in 7 pigment-containing bands and only a little free pigment. When thylakoid membranes were solubilized by different kinds of detergents and separated by SDS-PAGE, the same pigment-protein complexes had different absorption and fluorescence emission spectra. The absorption maxima of chlorophyll-a in PSI complexes were different in blue and red lights, and its short-wavelength fluorescence emission maxima at 77K also varied with detergent. PSI I complexes treated with DMG or DIG had absorption of fucoxinthin. The light-harvesting pigment-protein complexes (LHC) were also different in number, type and absorption spectrum. Two Chl a/c-protein complexes were resolved by SDS and four by DOC, but no fucoxinthin-Chl a/c-protein complexes (FCPC) were separated by SDS or DOC. DMG or DIG solubilization can yield three types of LHC, of which were two FCPC and one Chl a/c-protein complexes. According to the PAGE results, the best separation was obtained by using DMG or DIG, in which 6 pigment-protein complexes were resolved and a little free pigment were produced. |
| |
| Keywords: | Undaria pinnatifida Detergent SDS-PAGE Pigment-protein complexes Absorption spectra Fluorescence emissions spectra |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 |
