| 巨蛎属（Crassostrea）四种牡蛎精子Bindin蛋白海藻糖凝集素结构域（Fucose binding lectin）多样性研究 |
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| 引用本文： | 吴 琪,李 莉,张国范.巨蛎属（Crassostrea）四种牡蛎精子Bindin蛋白海藻糖凝集素结构域（Fucose binding lectin）多样性研究[J].海洋与湖沼,2011,42(2):251-255. |
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| 作者姓名： | 吴 琪 李 莉 张国范 |
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| 作者单位： | 中国科学院海洋研究所;中国科学院研究生院;中国科学院海洋研究所;中国科学院海洋研究所 |
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| 基金项目： | 国家自然科学基金重点项目, 40730845 号, 国家重点基础研究发展计划(973 项目)资助, 2010CB126401 号; 国家高技术研究发展计划(863 计划)资助, 2010AA1019004 号; 国家公益性行业(农业)科研专项, 3-53 号 |
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| 摘 要： | 提取巨蛎属中熊本牡蛎、葡萄牙牡蛎和日本巨牡蛎的基因组DNA,应用PCR技术扩增了三种牡蛎的海藻糖凝集素结构域（Fucose binding lectin,F-lectin）,结合GenBank中长牡蛎海藻糖凝集素结构域的序列利用邻接法对得到的序列进行了系统进化分析,并利用非同义替换率和同义替换率之间的比值分析了巨蛎属四...
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| 关 键 词： | 巨蛎属 结合素蛋白 海藻糖凝集素结构域 多样性 正选择位点 |
| 收稿时间： | 2010/4/13 0:00:00 |
| 修稿时间： | 2010/6/10 0:00:00 |
STUDY ON SPERM BINDIN FUCOSE-BINDING LECTIN OF FOUR SPECIES OF CRASSOSTREA |
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| WU Qi,LI Li and ZHANG Guo-Fan.STUDY ON SPERM BINDIN FUCOSE-BINDING LECTIN OF FOUR SPECIES OF CRASSOSTREA[J].Oceanologia Et Limnologia Sinica,2011,42(2):251-255. |
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| Authors: | WU Qi LI Li and ZHANG Guo-Fan |
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| Institution: | Institute of Oceanology, Chinese Academy of Sciences;Graduate School, Chinese Academy of Sciences;Institute of Oceanology, Chinese Academy of Sciences;Institute of Oceanology, Chinese Academy of Sciences |
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| Abstract: | Gamete recognition proteins (GRPs) play an important role in recognition between sperm and egg. Bindin is a major protein packaged as insoluble granules in sperm acrosomes. Fucose binding lectin (F-lectin) is the main functional domain of bindin. F-lectin repeats of Crassostrea sikamea, C. angulata and C. nippona have been got by polymerase chain reaction (PCR). NJ tree is constructed to analyze the relationship of eleven F-lectin repeats from C. sikamea, C. angulata, C. nippona and C. gigas. Five positive selected positions (40, 66, 67, 123 and 125) are identified among eleven haplotypes of four species. They are all located on the F-lectin binding face around the three recognition motif residues (H37, R64 and R70). We assume that these five clustered amino acids are related with species specific recognition. |
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| Keywords: | Crassostrea Bindin Fucose-binding lectin Polymorphism Positive selection |
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