Spatial distribution, diversity and composition of bacterial communities in sub-seafloor fluids at a deep-sea hydrothermal field of the Suiyo Seamount |
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| Authors: | Shingo Kato Kurt Hara Hiroko Kasai Takashi Teramura Michinari Sunamura Jun-ichiro Ishibashi Takeshi Kakegawa Toshiro Yamanaka Hiroyuki Kimura Katsumi Marumo Tetsuro Urabe Akihiko Yamagishi |
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| Institution: | 1. Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan;2. Earth and Planetary Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;3. Department of Earth Planetary Sciences, Faculty of Science, Kyushu University, 6-101 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;4. Graduate School of Science, Tohoku University, Aramaki-aza-aoba, Sendai, Miyagi 980-8952, Japan;5. Graduate School of Natural Science and Technology, Okayama University, 1-1, Naka 3-chome, Tsushima, Okayama 700-8530, Japan;6. Department of Geosciences, Faculty of Science, Shizuoka University, 836 Oya, Suruga-ku, Shizuoka 422-8529, Japan;7. Institute for Geology and Geoinformation, National Institute of Advanced Industrial Science and Technology, Central 7, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567, Japan |
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| Abstract: | Spatial distribution, diversity, and composition of bacterial communities within the shallow sub-seafloor at the deep-sea hydrothermal field of the Suiyo Seamount, Izu-Bonin Arc, Western Pacific Ocean, were investigated. Fluids were sampled from four boreholes in this area. Each borehole was located near or away from active vents, the distance ranging 2–40 m from active vents. In addition, fluids discharging from a natural vent and ambient seawater were sampled in this area. We extracted DNA from each sample, amplified bacterial 16S rRNA genes by PCR, cloned the PCR products and sequenced. The total number of clones analyzed was 348. Most of the detected phylotypes were affiliated with the phylum Proteobacteria, of which the detection frequency in each clone library ranged from 84.6% to 100%. The bacterial community diversity and composition were different between hydrothermal fluids and seawater, between fluids from the boreholes and the vent, and even among fluids from each borehole. The relative abundances of the phylotypes related to Thiomicrospira, Methylobacterium and Sphingomonas were significantly different among fluids from each borehole. The phylotypes related to Thiomicrospira and Alcanivorax were detected in all of the boreholes and vent samples. Our findings provide insights into bacterial communities in the shallow sub-seafloor environments at active deep-sea hydrothermal vent fields. |
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| Keywords: | Bacteria 16S ribosomal RNA gene Deep-sea hydrothermal vent Sub-seafloor Suiyo Seamount Izu-Bonin Island Arc Japan |
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