| Isolation and identification of a marine killer yeast strain YF07b and cloning of the gene encoding killer toxin from the yeast |
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| 作者姓名： | WANG Xianghong CHI Zhenming LI Jing |
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| 作者单位： | WANG Xianghong1，CHI Zhenming1，LI Jing1 1.UNESCO Chinese Center of Marine Biotechnology，Ocean University of China，Qingdao 266003，China |
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| 摘 要： | It was found that the marine yeast strain YF07b could secrete a large amount of killer toxin against a pathogenic yeast strain WCY which could cause milky disease in Portunus trituberculatus.The marine yeast strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences.The gene encoding killer toxin in the marine yeast strain YF07b was amplified by PCR technology.After sequencing,the results show that an open reading frame,consisting of 1 281 bp,encoded a presumed protein of 427 amino acids.The sequence of the cloned gene was found to have 99% match with that of the gene encoding killer toxin in Pichia anomalas strain K.A signal peptide including 17 amino acids appeared in the N-terminal domain of the killer toxin.Therefore,the mature protein consisted of 410 amino acids,its molecular mass was estimated to be 47.4 ku and its isoelctronic point was 4.5.
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| 关 键 词： | 致病酵母 螃蟹 毒素 基因编码 海洋细菌 |
| 收稿时间： | 2007-05-14 |
| 修稿时间： | 2007/9/10 0:00:00 |
Isolation and identification of a marine killer yeast strain YF07b and cloning of the gene encoding killer toxin from the yeast |
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| WANG Xianghong,CHI Zhenming,LI Jing.Isolation and identification of a marine killer yeast strain YF07b and cloning of the gene encoding killer toxin from the yeast[J].Acta Oceanologica Sinica,2007,26(6):101-108. |
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| Authors: | WANG Xianghong CHI Zhenming and LI Jing |
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| Institution: | UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao 266003, China |
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| Abstract: | It was found that the marine yeast strain YF07b could secrete a large amount of killer toxin against a pathogenic yeast strain WCY which could cause milky disease in Portunus trituberculatus.The marine yeast strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences.The gene encoding killer toxin in the marine yeast strain YF07b was amplified by PCR technology.After sequencing,the results show that an open reading frame,consisting of 1 281 bp,encoded a presumed protein of 427 amino acids.The sequence of the cloned gene was found to have 99% match with that of the gene encoding killer toxin in Pichia anomalas strain K.A signal peptide including 17 amino acids appeared in the N-terminal domain of the killer toxin.Therefore,the mature protein consisted of 410 amino acids,its molecular mass was estimated to be 47.4 ku and its isoelctronic point was 4.5. |
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| Keywords: | pathogenic yeast crab marine killer yeast identification killer toxin gene cloning |
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