| 光照和盐度对浒苔类胡萝卜素生物合成的影响 |
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| 引用本文： | 何渊,叶元土,沈颂东.光照和盐度对浒苔类胡萝卜素生物合成的影响[J].海洋学报(英文版),2020,39(10):50-57. |
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| 作者姓名： | 何渊 叶元土 沈颂东 |
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| 作者单位： | 苏州大学基础医学与生物科学学院 |
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| 基金项目： | the Jiangsu Planned Projects for Postdoctoral Research Funds;The National Key R&D Program of China under contract;the Priority Academic Program Development of Jiangsu Higher Education Institutions;the MNR Key Laboratory of Eco-Environmental Science and Technology,China under contract |
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| 摘 要： | 浒苔是一种在“绿潮”灾害中主要参与的绿藻。类胡萝卜素的生物合成是一种维持藻类正常生命活动非常重要的基本的萜类代谢活动。在本研究中，我们首次报告了浒苔2-C-甲基-D-赤藓糖醇4-磷酸（MEP）途径中所有基因的完整序列，这是浒苔中唯一的类胡萝卜素合成途径。随后，我们将这些基因序列与其他物种进行了比较。此外，通过检测三种不同的光照（1 000 lx，5 000 lx和12000 lx）和盐度（12‰，24‰和40‰）培养条件下的类胡萝卜素含量和参与此代谢的关键基因的表达水平差异，我们发现浒苔类胡萝卜素的合成可能受光照和盐度的影响，低光照和高盐度可以促进类胡萝卜素的合成。本研究结果表明：浒苔MEP途径和下游途径中涉及的基因可以在分子水平影响其类胡萝卜素的生物合成。本研究有助于更好地了解这些参与浒苔类胡萝卜素生物合成基因的作用以及环境对这些基因的调控。
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| 关 键 词： | 浒苔 类胡萝卜素 MEP途径 光照 盐度 |
| 收稿时间： | 2019/11/22 0:00:00 |
Effects of light and salinity on carotenoid biosynthesis in Ulva prolifera |
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| He Yuan,Ye Yuantu,Shen Songdong.Effects of light and salinity on carotenoid biosynthesis in Ulva prolifera[J].Acta Oceanologica Sinica,2020,39(10):50-57. |
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| Authors: | He Yuan Ye Yuantu Shen Songdong |
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| Institution: | College of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China |
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| Abstract: | Ulva prolifera is a green alga that plays an important role in green tides. Carotenoid biosynthesis is a basic terpenoid metabolism that is very important for maintaining normal life activities in algae. In this study, we first reported the complete sequences of all genes in the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, which is the only carotenoid synthesis pathway in U. prolifera. Then, we compared these genes with those of other species. Additionally, by detecting the carotenoid contents and expression levels of key genes participating in carotenoid biosynthesis in U. prolifera under three different light (1 000 lx, 5 000 lx and 12 000 lx) and salinity (12, 24 and 40) regimes, we found that carotenoid synthesis could be influenced by light and salinity, such that low light and high salinity could promote the synthesis of carotenoids. The results showed that the expression levels of genes involved in the MEP and the downstream pathway could affect the biosynthesis of carotenoids at the molecular level. This study contributes to a better understanding of the roles of genes participating in carotenoid biosynthesis in U. prolifera and the environmental regulation of these genes. |
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| Keywords: | U prolifera carotenoid MEP pathway light salinity |
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