| 强光照和黑暗条件下刺参体壁的基因表达差异 |
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| 引用本文： | 张立斌,冯其明,孙丽娜,房燕,徐冬雪,张涛,杨红生.强光照和黑暗条件下刺参体壁的基因表达差异[J].海洋学报(英文版),2018,37(5):54-66. |
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| 作者姓名： | 张立斌 冯其明 孙丽娜 房燕 徐冬雪 张涛 杨红生 |
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| 作者单位： | 中国科学院海洋生态与环境科学重点实验室(中国科学院海洋研究所);青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室,中国科学院海洋生态与环境科学重点实验室(中国科学院海洋研究所);中国科学院大学,中国科学院海洋生态与环境科学重点实验室(中国科学院海洋研究所);青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室,鲁东大学农学院,青岛农业大学海洋科学与工程学院,中国科学院海洋生态与环境科学重点实验室(中国科学院海洋研究所);青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室,中国科学院海洋生态与环境科学重点实验室(中国科学院海洋研究所);青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室 |
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| 基金项目： | The National Natural Science Foundation of China under contract No. 41676136; the Strategic Priority Research Program of the Chinese Academy of Sciences under contract No. XDA11020703; the Science and Technology Major Project of Shandong Province (Industry Transformation and Upgrading) under contract No. 2015ZDZX05002. |
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| 摘 要： | 参对光照变化非常敏感,研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况,通过“对照”与“黑暗”,“对照”与“强光”和“黑暗”与“强光”的比较,在|log2 ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明,“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多,而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对,发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。
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| 关 键 词： | 海参 刺参 基因表达 黑暗 光照 体壁 |
| 收稿时间： | 2017/1/25 0:00:00 |
| 修稿时间： | 2017/3/9 0:00:00 |
Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions |
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| ZHANG Libin,FENG Qiming,SUN Lin,FANG Yan,XU Dongxue,ZHANG Tao and YANG Hongsheng.Differential gene expression in the body wall of the sea cucumber (Apostichopus japonicus) under strong lighting and dark conditions[J].Acta Oceanologica Sinica,2018,37(5):54-66. |
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| Authors: | ZHANG Libin FENG Qiming SUN Lin FANG Yan XU Dongxue ZHANG Tao and YANG Hongsheng |
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| Institution: | 1.CAS Key laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology,Chinese Academy of Sciences,Qingdao,China;2.Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao,China;3.University of Chinese Academy of Sciences,Beijing,China;4.School of Agriculture,Ludong University,Yantai,China;5.College of Marine Science and Engineering,Qingdao Agricultural University,Qingdao,China |
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| Abstract: | Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light (“light”), normal light (“control”) and fully dark (“dark”) environment. In the comparisons of “control” vs. “dark”, ”control” vs. “light” and “dark” vs. “light”, 1 161, 113 and 1 705 differentially expressed genes (DEGs) were identified following the criteria of |log2ratio|≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that “cellular process” and “binding” enriched the most DEGs in the category of “biological process” and “molecular function”, while “cell” and “cell part” enriched the most DEGs in the category of “cellular component”. And the DEGs were mapped to 214, 41 and 229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light. |
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| Keywords: | sea cucumber Apostichopus japonicus gene expression dark light body wall |
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