| 太平洋深海沉积物来源的菌株Tenacibaculum sp.HMG1对孔雀石绿降解特性的研究 |
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| 引用本文： | 曲武,洪国粦,赵晶.太平洋深海沉积物来源的菌株Tenacibaculum sp.HMG1对孔雀石绿降解特性的研究[J].海洋学报(英文版),2018,37(6):104-111. |
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| 作者姓名： | 曲武 洪国粦 赵晶 |
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| 作者单位： | 厦门大学生命与科学学院,厦门大学附属第一医院检验医学部,厦门大学海洋与地球学院 |
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| 基金项目： | The Scientific Research Project of Xiamen Southern Oceanographic Center under contract No. 17GZP007NF03; the China Ocean Mineral Resources R&D Association under contract No. DY-125-22-QY-18. |
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| 摘 要： | 本文在太平洋深海沉积物中分离得到一株孔雀石绿降解菌株,鉴定命名为Tenacibaculum sp.HMG1。通过菌株生长实验和高效液相色谱的研究表明, HMG1菌株可以在20 mg/L的孔雀石绿中维持较快的生长速率,并且在12 h内可降解98.8%的孔雀石绿,这证明该菌株具有很高的孔雀石绿耐受能力和降解活性。通过基因组测序在HMG1菌株发现一条过氧化物酶基因可能参与了孔雀石绿的降解,随后利用原核表达获得了相应的重组蛋白。实验表明,该重组过氧化物酶具有极强的活性,可在1000 mg/L的孔雀石绿中发挥降解功能。本文利用液相色谱-质谱联用（LC-MS）技术对孔雀石绿的菌株降解产物和重组酶降解产物进行鉴定,并基于鉴定结果推测了两种降解途径。结果发现两种降解方式存在共同的降解途径。此外,孔雀石绿降解条件的实验结果证明重组过氧化物酶可以在低温（20℃）、复杂的pH值（6.0–9.0）、高盐度（100 mmol/L）、金属离子和EDTA等反应条件下依旧维持很高的孔雀石绿降解活性。以上实验结果表明,HMG1菌株和重组过氧化物酶均在孔雀石绿污染生物修复方面具有很大潜力。
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| 关 键 词： | 深海沉积物 粘着杆菌 过氧化物酶 孔雀石绿降解特性 |
| 收稿时间： | 2017/9/18 0:00:00 |
| 修稿时间： | 2017/12/12 0:00:00 |
Degradation of malachite green dye by Tenacibaculum sp. HMG1 isolated from Pacific deep-sea sediments |
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| QU Wu,HONG Guolin and ZHAO Jing.Degradation of malachite green dye by Tenacibaculum sp. HMG1 isolated from Pacific deep-sea sediments[J].Acta Oceanologica Sinica,2018,37(6):104-111. |
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| Authors: | QU Wu HONG Guolin and ZHAO Jing |
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| Institution: | 1.School of Life Sciences, Xiamen University, Xiamen 361005, China2.Department of Laboratory Medicine, First Affiliated Hospital, Xiamen University, Xiamen 361005, China3.College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China |
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| Abstract: | A deep-sea bacterium from the Pacific Ocean identified as Tenacibaculum sp. HMG1 was found to have strong malachite green (MG) degradation activity. The MG tolerance and decolorizing activities of strain HMG1 were confirmed by bacterial growth and high-performance liquid chromatography (HPLC) analyses. Strain HMG1 was capable of removing 98.8% of the MG in cultures within 12 h and was able to grow vigorously at 20 mg/L MG. A peroxidase gene detected in the genome of strain HMG1 was found to be involved in the MG biodegradation process. The corresponding recombinant peroxidase (rPOD) demonstrated high degradative activity at 1 000 mg/L MG. Based on the common candidate intermediates, strain HMG1 was inferred to have one primary MG degradation pathway containing rPOD. In addition, five other candidate intermediates of the rPOD-MG degradative process were detected. The optimal conditions for MG degradation were determined and showed that strain HMG1 and the rPOD enzyme could maintain high bioactivity at a low temperature (20°C), variable pH values (6.0–9.0), higher salinities (100 mmol/L) and other factors, such as multiple metal ions, H2O2 and EDTA. MG-tolerant strain Tenacibaculum sp. HMG1 and its peroxidase have prospective applications as environmental amendments for MG degradation during coastal remediation. |
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| Keywords: | deep-sea sediment Tenacibaculum mesophilum HMG1 peroxidase malachite green degradation characteristics |
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