| 西太平洋深海海水中乙醛降解菌多样性及群落结构研究 |
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| 引用本文： | 高波良,商谢谢,李丽,狄文婕,曾润颖.西太平洋深海海水中乙醛降解菌多样性及群落结构研究[J].海洋学报(英文版),2018,37(8):54-64. |
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| 作者姓名： | 高波良 商谢谢 李丽 狄文婕 曾润颖 |
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| 作者单位： | 厦门大学生命科学学院, 中国厦门 361102,厦门大学生命科学学院, 中国厦门 361102,国家海洋局第三研究所国家海洋局海洋生物遗传资源重点实验室, 中国厦门 361005,国家海洋局第三研究所国家海洋局海洋生物遗传资源重点实验室, 中国厦门 361005,国家海洋局第三研究所国家海洋局海洋生物遗传资源重点实验室, 中国厦门 361005;福建省海洋生物资源开发利用协同创新中心, 中国厦门 36100 |
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| 基金项目： | The Xiamen Ocean Economic Innovation and Development Demonstration Project under contract No. 16PZP001SF16. |
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| 摘 要： | As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.
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| 关 键 词： | 乙醛降解菌 ALDH 深海环境 原位富集 |
| 收稿时间： | 2017/12/14 0:00:00 |
| 修稿时间： | 2018/3/13 0:00:00 |
Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean |
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| GAO Boliang,SHANG Xiexie,LI Li,DI Wenjie and ZENG Runying.Phylogenetically diverse, acetaldehyde-degrading bacterial community in the deep sea water of the West Pacific Ocean[J].Acta Oceanologica Sinica,2018,37(8):54-64. |
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| Authors: | GAO Boliang SHANG Xiexie LI Li DI Wenjie and ZENG Runying |
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| Institution: | 1.School of Life Sciences, Xiamen University, Xiamen 361102, China2.State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China3.State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China |
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| Abstract: | As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study, acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16S rRNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source, and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria, Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different. Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant (63.5% clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ- Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus (45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas (9% in situ enriched sample) and Streptococcus (6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas, Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12, belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants. |
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| Keywords: | acetaldehyde-degrading bacteria ALDH deep sea in situ |
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