| Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers |
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| 作者姓名： | CHANG Yumei DING Lei LI Mingyun XUE Liangyi LIANG Liqun HE Jianguo LEI Qingquan |
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| 作者单位： | [1]Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070,China [2]College of Life Sciences, Zhongshan University, Guangzhou 510275, China [3]Life Science of Technology Institute, Dalian Fisheries University, Dalian 116023, China [4]Life Science and Biotechnology Faculty, Ningbo University, Ningbo 315211, China [5]College of Electrical ＆ Electronic Engineering, Harbin University of Science and Technology, Harbin 150080, China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | The germplasm of breeding large yellow croaker （Pseudosciaena crocea Richardson） was revealed using 12 microsatellite markers. The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min - Yue stocks and two hybrid groups, as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity. The value of pair- wise differentiation coefficient（ FST ） was only 13. 1% between Daiqu and Min -Yue stocks, demonstrating the low level of differentiation and a close relationship. However, STRUCTURE simulations and phylogenetic tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures. Examinations of individual admixture showed that Min - Yue stock had been contaminated by alien individuals. Moreover, the genetic structures of the two hybrid groups resembled those of their parents, especially affected more by their female parents. Finally, the values of average observed heterozygosity between parents and their ascendants were compared and tested, as a result of no detectable differences （P 〉 0. 05）.
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| 关 键 词： | 海洋 生物种群 杂交 遗传多样性 |
| 收稿时间： | 2007/10/12 0:00:00 |
| 修稿时间： | 4/9/2008 12:00:00 AM |
Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers |
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| CHANG Yumei,DING Lei,LI Mingyun,XUE Liangyi,LIANG Liqun,HE Jianguo,LEI Qingquan.Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers[J].Acta Oceanologica Sinica,2008,27(4):156-164. |
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| Authors: | CHANG Yumei DING Lei LI Mingyun XUE Liangyi LIANG Liqun HE Jianguo and LEI Qingquan |
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| Institution: | Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;College of Life Sciences, Zhongshan University, Guangzhou 510275, China;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;Life Science of Technology Institute, Dalian Fisheries University, Dalian 116023, China;Life Science and Biotechnology Faculty, Ningbo University, Ningbo 315211, China;Life Science and Biotechnology Faculty, Ningbo University, Ningbo 315211, China;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin 150080, China;College of Life Sciences, Zhongshan University, Guangzhou 510275, China;College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin 150080, China |
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| Abstract: | The germplasm of breeding large yellow croaker(Pseudosciaena crocea Richardson)was revealed using 12 microsatellite markers.The results showed that the genetic diversities were on a mediated level in the bred Daiqu and Min-Yue stocks and two hybrid groups,as represented by 4.83 of the mean number of alleles and 0.561 of the average observed heterozygosity.The value of pair-wise differentiation coefficient(Fst)was only 13.1% between Daiqu and Min-Yue stocks,demonstrating the low level of differcn-tiation and a close relationship.However,STRUCTURE simulations and phylogenetie tree based on the UPGMA method supported that they are geographically different populations of the same species with distinct genetic structures.Examinations of individual ad-mixture showed that Min-Yue stock had been contaminated by alien individuals.Moreover,the genetic structures of the two hybridgroups resembled those of their parents,especially affected more by their female parents.Finally,the values of average observed beterozygasity between parents and their ascendants were compared and tested,as a result of no detectable differences(P>0.05). |
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| Keywords: | Pseudosciaena crocea microsatellite marker bred stock germplasm adnlix |
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