| Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4 |
| |
| 作者姓名： | MA Yuexin LIU Pengliang ZHANG Yongsheng CAO Shanmao LI Dantong CHEN Wei |
| |
| 作者单位： | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China |
| |
| 基金项目： | The Natural Science Foundation of Liaoning Province under contract No. 20062129 |
| |
| 摘 要： | The e?ect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa
was assayed and it was found that the bacterial bioˉlm and cell-free supernatant strongly inhibited
spore germination. In attempts to deˉne the chemical nature of the antifouling substance in the
supernatant of CI4, the culture supernatants were tested for activity after heat treatment, enzy-
matic treatments, size fractionation, and separation into aqueous and organic fractions. Results
suggest that this bacterium produces an extracellular component with speciˉc activity toward algal
spores that was heat-sensitive and between 3 and 10 kDa in molecular size. The exposure of the
organic phase fraction to spores showed inhibitive e?ect on spore germination. Pronase and car-
boxypeptidase y did not signiˉcantly a?ect the activity of inhibitory component, suggesting that the
component was not a protein or a peptide. The bacterium CI4 was identiˉed as Pseudoalteromonas
haloplanktis based on the phenotypic characters and 16S rRNA gene analysis.
|
| 关 键 词： | 孢子萌发 海洋细菌 孔石莼 单胞 rRNA基因 羧肽酶Y 细菌生物膜 化学性质 |
| 收稿时间： | 17 April 2007 |
| 修稿时间： | 2009/8/19 0:00:00 |
Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4 |
| |
| MA Yuexin,LIU Pengliang,ZHANG Yongsheng,CAO Shanmao,LI Dantong,CHEN Wei.Inhibition of spore germination of Ulva pertusa by the marine bacterium Pseudoalteromonas haloplanktis CI4[J].Acta Oceanologica Sinica,2010,29(1):69-78. |
| |
| Authors: | MA Yuexin LIU Pengliang ZHANG Yongsheng CAO Shanmao LI Dantong and CHEN Wei |
| |
| Institution: | Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Fisheries University, Dalian 116023, China |
| |
| Abstract: | The effect of the bacterial strain CI4 on the germination of spores from the green alga Ulva pertusa was assayed and it was found that the bacterial biofilm and cell-free supernatant strongly inhibited spore germination. In attempts to define the chemical nature of the antifouling substance in the supernatant of CI4,the culture supernatants were tested for activity after heat treatment,enzymatic treatments,size fractionation,and separation into aqueous and organic fractions. Results suggest that this bacter... |
| |
| Keywords: | Pseudoalteromonas antifouling bacterium Ulva pertusa biofouling anti-algal activity |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《海洋学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《海洋学报(英文版)》下载免费的PDF全文 |
