| 温度和铜离子浓度对近江牡蛎超氧化物岐化酶活力的联合效应 |
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| 引用本文： | 王辉,杨洪帅,刘加慧,李艳红,刘志刚.温度和铜离子浓度对近江牡蛎超氧化物岐化酶活力的联合效应[J].海洋学报(英文版),2016,35(4):51-57. |
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| 作者姓名： | 王辉 杨洪帅 刘加慧 李艳红 刘志刚 |
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| 作者单位： | 淮阴师范学院生命科学学院, 淮安 223300, 中国,广东海洋大学水产学院, 湛江 524088, 中国,广东海洋大学水产学院, 湛江 524088, 中国,广东海洋大学水产学院, 湛江 524088, 中国,广东海洋大学水产学院, 湛江 524088, 中国 |
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| 基金项目： | The Guangdong Province Education Department under contract No.GCZX-A0909; the Guangdong Province Ocean and Fisheries Science & Technology Extension Project under contract No.20120980; the Guangdong Province Industry-University-Science Partnership Project under contract No.20110908; the Science & Technology Project of Huaiyin Normal University under contract No.WH0031. |
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| 摘 要： | Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18–34°C) and copper ion concentration(0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P0.01), the quadratic effect of temperature was significant(P0.05); the linear effect of copper ion concentration was not significant(P0.05), while the quadratic effect of copper ion concentration was highly significant(P0.01); the interactive effect of temperature and copper ion concentration was not significant(P0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.
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| 关 键 词： | 近江牡蛎 超氧化物岐化酶 温度 铜离子浓度 联合效应 |
| 收稿时间： | 2014/11/30 0:00:00 |
| 修稿时间： | 2015/9/14 0:00:00 |
Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis |
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| WANG Hui,YANG Hongshuai,LIU Jiahui,LI Yanhong and LIU Zhigang.Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis[J].Acta Oceanologica Sinica,2016,35(4):51-57. |
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| Authors: | WANG Hui YANG Hongshuai LIU Jiahui LI Yanhong and LIU Zhigang |
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| Institution: | 1.School of Life Sciences, Huaiyin Normal University, Huaian 223300, China2.Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China |
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| Abstract: | Superoxide dismutase (SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD (SOD1) and Mn-SOD (SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature (18–34°C) and copper ion concentration (0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant (P<0.01), the quadratic effect of temperature was significant (P<0.05); the linear effect of copper ion concentration was not significant (P>0.05), while the quadratic effect of copper ion concentration was highly significant (P<0.01); the interactive effect of temperature and copper ion concentration was not significant (P>0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator. |
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| Keywords: | Crassostrea ariakensis superoxide dismutase temperature copper ion concentration combined effect |
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