| 褐藻纲海带目Laminaria属和Saccharina属基于三个分子标记的系统发育分析 |
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| 作者姓名： | CHI Shan QIAN Hao LI Tianyong WANG Xumin LIU Cui REN Lei TANG Xuexi LIU Tao |
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| 作者单位： | Ocean University of China, Qingdao 266003, China;Ocean University of China, Qingdao 266003, China;Ocean University of China, Qingdao 266003, China;Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Chinese Academy of Sciences Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Ocean University of China, Qingdao 266003, China;Ocean University of China, Qingdao 266003, China;Ocean University of China, Qingdao 266003, China;Ocean University of China, Qingdao 266003, China |
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| 基金项目： | The National Natural Science Foundation of China under contract No. 41206116; the Postdoctoral Science Foundation of China under contract No. 2011M501167; the National Public Benefit Research Foundation of China under contract No. 201105021; the Fundamental Research Funds for the Central Universities under contract No. 201362040. |
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| 摘 要： | In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae–Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I(COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer(ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina.
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| 关 键 词： | 分子系统发育 分子标记 海带 线粒体基因组 系统发育分析 褐藻 采样序列 分类群 |
| 收稿时间： | 2/7/2013 12:00:00 AM |
| 修稿时间： | 2014/2/11 0:00:00 |
Phylogeny of genera Laminaria and Saccharina (Laminariales, Phaeophyceae) based on three molecular markers |
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| CHI Shan,QIAN Hao,LI Tianyong,WANG Xumin,LIU Cui,REN Lei,TANG Xuexi,LIU Tao.Phylogeny of genera Laminaria and Saccharina (Laminariales, Phaeophyceae) based on three molecular markers[J].Acta Oceanologica Sinica,2014,33(9):139-151. |
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| Authors: | CHI Shan QIAN Hao LI Tianyong WANG Xumin LIU Cui REN Lei TANG Xuexi and LIU Tao |
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| Institution: | 1.Ocean University of China, Qingdao 266003, China2.Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Chinese Academy of Sciences Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae-Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I (COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer (ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina. |
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| Keywords: | Saccharina Laminaria phylogeny COI rbcL ITS |
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