| Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides |
| |
| 作者姓名： | TIAN Chao WANG Guangce YE Naihao ZHANG Baoyu FAN Xiaolei ZENG Chengkui |
| |
| 作者单位： | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Graduate School of the Chinese Academy of Sciences, Beijing 100039, China [3]Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
| |
| 基金项目： | 国家自然科学基金，the Project of Scientific Innovation of Chinese Academy of Sciences under contract，the Project of Scientific Innovation of Institute of Oceanology，中国科学院资助项目，高比容电子铝箔的研究开发与应用项目 |
| |
| 摘 要： | 1 IntroductionRibulose-1,5-bisphosphatecarboxylase/oxygenase(EC 4.1.1.39;Rubisco) plays an important role in photo-synthetic carbon fixation by controlling the initial stageof the Calvin cycle and possessing the functions of bothCO2 fixation and ribulose-1, 5-bisphosphate (RuBP)oxygenation. Rubisco is composed of eight large andeight small subunits in higher plants and green algae,and the large subunit is encoded by chloroplast genomeand the small subunit by nuclear genome. The gene en-cod…
|
| 关 键 词： | 碳酸酵素 加氧酶 PCR 克隆技术 基因序列 光合作用 水生植物 |
| 收稿时间： | 2004-10-12 |
| 修稿时间： | 2005-03-03 |
Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides |
| |
| TIAN Chao,WANG Guangce,YE Naihao,ZHANG Baoyu,FAN Xiaolei,ZENG Chengkui.Cloning and sequence analysis of the partial sequence of the rbcL from Bryopsis hypnoides[J].Acta Oceanologica Sinica,2005,24(5):150-161. |
| |
| Authors: | TIAN Chao WANG Guangce YE Naihao ZHANG Baoyu FAN Xiaolei and ZENG Chengkui |
| |
| Institution: | 1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China3.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
| |
| Abstract: | The partial sequence of the rbcL from Bryopsis hypnoides, including the sequences of the upstream, extron and partial intron, was amplified by PCR and their sequences were determined. With Spinacia oleracea as the outgroup, neighbor-joining method and maximum parsimony method were used respectively to build phylogenetic trees according to the rbcL exon sequence among 13 species that were the typical species of six phyla. Two kinds of trees showed clearly that there were two groups among those species, the green lineage and the non-green lineage. And the relationships of algae in the green lineage were similar in the two trees but those in the non-green lineage were not consistent. Analysis of codon preference indicated that the codon preference of the rbcL exon of Bryopsis hypnoides distinctly differed from that of the relevant sequence of photosynthetic bacteria. |
| |
| Keywords: | rbcL Bryopsis hypnoides sequence analysis phylogenetic analysis codon preference |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《海洋学报(英文版)》下载免费的PDF全文 |
