Potential anticancer activity analysis of piscidin 5-like from Larimichthys crocea |
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| Authors: | Libing Zheng Jiayin Qiu Huihui Liu Changfeng Chi Longshan Lin |
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| Institution: | 1.National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China2.Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China |
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| Abstract: | Antitumor activity is one characteristic function of some certain antimicrobial peptides (AMPs) found in recent years. In the present study, we attempted to detect potential anticancer activity of a recombinant piscidin 5-like from Larimichthys crocea (rLc-P5L) which owned widely antibacterial and strong antiparasitic activity in vitro. The light microscope observation indicated rLc-P5L was of antitumor activity to HeLa cells, 293T cells and L929 cells. MTT assay showed the toxic sensitivity of rLc-P5L to three tumor cell strains was 293T>L929>HeLa. Scanning electron microscope (SEM) results showed rLc-P5L behaved like a lytic peptide to cause damage on cells membrane of L929 cells by forming globular clusters, even pores at 60 μmol/L, or degrading membrane to make it completely lose cytoskeleton structure at 80 μmol/L; rLc-P5L treatment also resulted in DNA degradation. Fluorescence observation results indicated rLc-P5L could cause L929 cells at least two obvious changes: one is nucleus, nuclear chromatin condensed in the margin, nuclear volume became smaller and shrank to be out of shape, or lysed to be debris; the other is cytoskeleton, they became disordered and polarized to make cells atrophic shapes, or even lysed to be debris. In summary, rLc-P5L owned potential anticancer activity causing membrane structure damage and genome DNA degradation. Interestingly, treatment with different concentration of rLc-P5L seemingly caused the similar but different changes, whether it indeed gave rise to cancer cells diverse death way, the further studies should be performed, and the detailed mechanisms were still need further explored. |
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| Keywords: | Larimichthys crocea piscidin 5-like anticancer activity membrane destruction DNA degradation |
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