| 大叶藻微粒体GST基因对温度胁迫响应的分子机制 |
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| 引用本文： | 闫文杰,刘娇,Seng Samphal,周斌,丁库克.大叶藻微粒体GST基因对温度胁迫响应的分子机制[J].海洋学报(英文版),2019,38(10):111-115. |
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| 作者姓名： | 闫文杰 刘娇 Seng Samphal 周斌 丁库克 |
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| 作者单位： | 中国海洋大学 海洋生命学院,中国科学院海洋研究所,柬埔寨皇家农业大学,中国海洋大学 海洋生命学院,中国疾病预防控制中心辐射防护与核安全医学所;中国疾病预防控制中心放射防护与核应急重点实验室 |
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| 基金项目： | The Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration under contract No. 201704; the Shandong Provincial Natural Science Foundation of China under contract No. ZR2018MD020. |
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| 摘 要： | 为了分析鳗草（Zostera marina）中微粒体谷胱甘肽S-转移酶在不同温度下的基因表达规律以及响应机制，本文通过在大肠杆菌中表达ZmGST，纯化重组蛋白以及热稳定性分析，以此为进一步阐述Z. marina的种群退化机制并提供理论依据。总之，鳗草中的微粒体GST的热稳定性和其对温度变化的响应决定了其对温度的耐受范围，并进而影响其恢复力。
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| 关 键 词： | 鳗草(Zostera marina)|抗氧化酶|谷胱甘肽S-转移酶(GST)|温度|酶活性 |
| 收稿时间： | 2018/5/20 0:00:00 |
Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina |
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| Yan Wenjie,Liu Jiao,Seng Samphal,Zhou Bin and Ding Kuke.Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina[J].Acta Oceanologica Sinica,2019,38(10):111-115. |
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| Authors: | Yan Wenjie Liu Jiao Seng Samphal Zhou Bin and Ding Kuke |
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| Institution: | Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China,Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China,Faculty of Fisheries & Aquaculture, Royal University of Agriculture, Phnom Penh 12101, Cambodia,Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China and National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China;Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China |
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| Abstract: | The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn. |
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| Keywords: | Zostera marina|antioxidant enzyme|glutathione S-transferase (GST)|temperature|enzyme activity |
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