| Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre |
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| 作者姓名： | WEI Manman ZHANG Rubing WANG Yuguang JI Houguo ZHENG Ji CHEN Xinhu ZHOU Hongbo |
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| 作者单位： | School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China;School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;Third Institute of Oceanography State, Oceanic Administration, Xiamen 361005, China;School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;Key Laboratory of Biometallurgy, Ministry of Education, Changsha 410083, China |
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| 基金项目： | Foundation item:The China Ocean Mineral Resources Research and Development Association under contract No.DYXM-115-02-2-07;the State Oceanic Administration of People's Republic of China under contract No.200805032;the National Natural Science Foundation of China under contract Nos 50621063 and 40646029. |
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| 摘 要： | The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields’ sediments along the Eastern Lau Spreading Centre (ELSC) in the Lau Basin (southwest Pacific). Microbial communities were surveyed by denatured gradient gel electrophoresis (DGGE) and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles separated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, representing two selected hydrothermal samples (19-4TVG8 and 19-4TVG11), were constructed. Twenty-three and 32 phylotypes were identified from 166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group Ⅰ and Ⅲ, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hydrothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.
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| 关 键 词： | 微生物群落结构 热液沉积物 热液喷口 深海热液 多样性 变性梯度凝胶电泳 rRNA基因 克隆鉴定 |
| 收稿时间： | 2011/5/19 0:00:00 |
| 修稿时间： | 2012/4/24 0:00:00 |
Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre |
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| WEI Manman,ZHANG Rubing,WANG Yuguang,JI Houguo,ZHENG Ji,CHEN Xinhu,ZHOU Hongbo.Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre[J].Acta Oceanologica Sinica,2013,32(2):42-51. |
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| Authors: | WEI Manman ZHANG Rubing WANG Yuguang JI Houguo ZHENG Ji CHEN Xinhua and ZHOU Hongbo |
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| Institution: | 1.School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China2.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China3.Third Institute of Oceanography State, Oceanic Administration, Xiamen 361005, China4.School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;Key Laboratory of Biometallurgy, Ministry of Education, Changsha 410083, China |
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| Abstract: | The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields’ sediments along the Eastern Lau Spreading Centre (ELSC) in the Lau Basin (southwest Pacific). Microbial communities were surveyed by denatured gradient gel electrophoresis (DGGE) and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles separated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, representing two selected hydrothermal samples (19-4TVG8 and 19-4TVG11), were constructed. Twenty-three and 32 phylotypes were identified from166 and 160 bacterial clones respectively, including Proteobacteria, Bacteroidetes, Firmicutes, Nitrospirae and Planctomycetes. The phylum Proteobacteria is dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group I and III, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hydrothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem. |
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| Keywords: | microbial diversity deep-sea hydrothermal vent DGGE 16S rRNA gene clone libraries |
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