| The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein |
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| 作者姓名： | LI Laihao XUE Changhu XUE Yong LI Zhaojie FU Xueyan |
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| 作者单位： | LI Laihao1，XUE Changhu 2*，XUE Yong 2，LI Zhaojie 2，FU Xueyan 21. South China Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510300，China 2. Department of Food Science and Technology，Ocean University of China，Qingdao 266003，China |
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| 基金项目： | 国家自然科学基金;广东省自然科学基金 |
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| 摘 要： | 1Introduction Thegeneration,reactionandconsequenceof freeradicalsinbiologicalsystemshavereceived muchattentionrecentlyinconnectionwithavariety ofpathologicalevents(BarryandSusanna,1993;FangandLi,1999).LDLoxidizedbyexposureto tracemetalionsCu2 andFe3 (Hein…
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| 关 键 词： | 昆布属植物 岩藻依聚糖 脂蛋白 抗氧剂 |
| 收稿时间： | 2006-02-12 |
| 修稿时间： | 2006-05-30 |
The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein |
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| LI Laihao,XUE Changhu,XUE Yong,LI Zhaojie,FU Xueyan.The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein[J].Acta Oceanologica Sinica,2006,25(4):124-130. |
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| Authors: | LI Laihao XUE Changhu XUE Yong LI Zhaojie and FU Xueyan |
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| Institution: | 1.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China2.Department of Food Science and Technology, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Five fucoidan fractions from Laminaria japonica with different sulfate content and molecular weight were prepared by anion-exchange chromatography and mild acid hydrolysis. Their antioxidant effects on azo radicals 2-2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) induced oxidation of human low-density lipoprotein (LDL) were evaluated by monitoring cholesteryl ester hydroperoxides (CHL-OOH) formation kinetics through reverse-phase high performance liquid chromatography (RP-HPLC) analysis. Fucoidan F-C with a low molecular mass of 2 000～8 000 and a sulfate content of 24.3% had much stronger protective antioxidant effect than other four fucoidan fractions on the hydrophilic radical AAPH induced LDL oxidation. However, the highly sulfated fucoidan fraction L-B with a molecular mass of 20 000 was completely ineffective in protecting LDL from the AAPH induced oxidation. The results suggested that both molecular mass and sulfate content of fucoidan played very important roles in their effects on the AAPH induced LDL oxidation. |
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| Keywords: | Laminaria japonica fucoidan fraction low-density lipoprotein antioxidant cholesteryl ester hydroperoxide |
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