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Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry |
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| Authors: | HOU Jianjun LAI Hongyan HUANG Bangqin CHEN Jixin College of Fisheries Huazhong Agricultural University Wuhan China College of Life Sciences Hubei Normal University Huangshi China State Key Laboratory of Marine Environmental Science Environmental Science Research Center Xiamen University Xiamen China Key Laboratory of Freshwater Fish Germplasm Resources Biotechnology Yangtse River Fisheries Research Institute Jinzhou China State Key La |
| Institution: | [1]College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China [2]College of Life Sciences, Hubei Normal University, Huangshi 435002, China [3]State Key Laboratory of Marine Environmental Science, Environmental Science Research Center Xiamen University, Xiamen 361005, China [4]Key Laboratory of Freshwater Fish Germplasm Resources and Biotechnology, Yangtse River Fisheries Research Institute, Jinzhou 434000, China [5]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
| Abstract: | Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes (DNA probes) were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with flu... |
| Keywords: | oligonucleotide DNA probes Prorocentrum minimum Takayama pulchella fluores-cence in situ hybridization flow cytometry |
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