| Crystallographic and NMR studies on sterols from green alga Chaetomorpha basiretorsa Setchell |
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| 作者姓名： | SHI Dayong XU Feng HE Juan SHI Jiangong FAN Xiao HAN Lijun |
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| 作者单位： | SHI Dayong（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ;
XU Feng（Affiliated Hospital of Medical College,Qingdao University,Qingdao 266003,China） ;
HE Juan （Affiliated Hospital of Medical College,Qingdao University,Qingdao 266003,China） ;
SHI Jiangong（Institute of Materia Medica,Chinese Academy of Medical Sciences and Beijing Union Medical College,Beijing 100050,China） ;
FAN Xiao（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ;
HAN Lijun（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ; |
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| 基金项目： | The State "863" High Teeh Program of China under contract Nes 2007AA09Z410 and 2007AA091604 and Key Innovative Project of the Chinese Academy of Sciences under contract No.KZCX2-YW-209. |
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| 摘 要： | Chemical investigation of the ethanol extract of the marine green alga Chaetomorpha basiretorsa Setchell led to the isolation of a new sterol stigmast-4,28-dien-3α,6β-diol 1 in addition to the five known sterols of β-lawsaritol 2, saringosterol 3, 24-hydroperoxy-24-vinyl - cholesterol 4, β-stigmasterol 5, 29-hydroxystigmasta-5, 24 （28） -dien-3β-ol 6. Compounds were isolated by normal phase silica gel and Sephadex LH - 20 gel colum chromatography, reverse phase HPLC and recrystalization. Their structures were elucidated by spectroscopic methods including MS, IR 1D/2D NMR and X-ray analysis. Cytotoxicity of compounds was screened by using the standard MTF method. All these compounds were isolated from the green alga Chaetomorpha basiretorsa Setchell for the first time and they were inactive （50% inhibitory concentration was greater than 10 μg/cm^3） against KB, Bel -7402, PC - 3M, Ketr 3 and MCF-7 cell lines.
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| 关 键 词： | 绿藻 固醇 X射线晶体结构 核磁共振 |
| 收稿时间： | 2006/11/17 0:00:00 |
| 修稿时间： | 2007/6/20 0:00:00 |
Crystallographic and NMR studies on sterols from green alga Chaetomorpha basiretorsa Setchell |
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| SHI Dayong,XU Feng,HE Juan,SHI Jiangong,FAN Xiao,HAN Lijun.Crystallographic and NMR studies on sterols from green alga Chaetomorpha basiretorsa Setchell[J].Acta Oceanologica Sinica,2008,27(1):147-151. |
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| Authors: | SHI Dayong XU Feng HE Juan SHI Jiangong FAN Xiao and HAN Lijun |
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| Institution: | 1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China2.Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China3.Institute of Materia Medica, Chinese Academy of Medical Sciences and Beijing Union Medical College, Beijing 100050, China |
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| Abstract: | Chemical investigation of the ethanol extract of the marine green alga Chaetomorpha basiretorsa Setchell led to the isolation of a new sterol stigrnast-4,28-dien-3α,6β-diol 1 in addition to the five known sterols of β-1awsaritol 2,saringosterol 3,24-hydroper- oxy-24-vinyl-cholesterol 4,β-stigmasterol 5,29-hydroxystigmasta-5,24(28)-dien-3β-ol 6.Compounds were isolated by normal phase silica gel and Scphadex LH-20 gel colum chromatography,reverse phase HPLC and recrystalization.Their structures were elucidated by spectroscopic methods including MS,IR 1D/2D NMR and X-ray analysis.Cytotoxicity of compounds was screened by using the standard MTT method.All these compounds were isolated from the green alga Chaetomorpha basiretorsa Setchell for the first time and they were inactive (50% inhibitory concentration was greater than 10 μg/cm3) against KB,Bel-7402,PC- 3M,Ketr 3 and MCF-7 cell lines. |
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| Keywords: | green alga sterol X-ray crystal structure |
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