| Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis |
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| 作者姓名： | XIAO Haidong CHEN Changsheng XU Yan JI Dehu XIE Chaotian |
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| 作者单位： | Fisheries College, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China;Fisheries College, Jimei University, Xiamen 361021, China |
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| 基金项目： | The National Natural Science Foundation of China under contract Nos 41176151 and 41276177; the National High Technology Research & Development Program of China under contract No. 2012AA100811; the Funds for Distinguished Young Scientists of Fujian Province of China under contract No. 2010J06016. |
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| 摘 要： | Fructose-1,6-bisphosphatase(FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends(RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5′ untranslated region(UTR) of 92 bp, a 3′?UTR of 69 bp, and an open reading frame(ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.
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| 关 键 词： | 酶基因 叶绿体 紫菜 表达分析 果糖 克隆 二磷酸 丝状噬菌体 |
| 收稿时间： | 3/6/2013 12:00:00 AM |
| 修稿时间： | 2013/4/28 0:00:00 |
Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis |
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| XIAO Haidong,CHEN Changsheng,XU Yan,JI Dehu,XIE Chaotian.Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis[J].Acta Oceanologica Sinica,2014,33(4):92-100. |
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| Authors: | XIAO Haidong CHEN Changsheng XU Yan JI Dehua and XIE Chaotian |
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| Institution: | Fisheries College, Jimei University, Xiamen 361021, China |
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| Abstract: | Fructose-1,6-bisphosphatase (FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends (RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5' untranslated region (UTR) of 92 bp, a 3' UTR of 69 bp, and an open reading frame (ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress. |
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| Keywords: | Pyropia haitanensis fructose-1 6-bisphosphatase gene cloning qRT-PCR RACE |
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