| Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing |
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| 作者姓名： | LIU Yongjian YANG Guanpin GUAN Xiaojing MEN Rongxin |
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| 作者单位： | LIU Yongjian1，YANG Guanpin1*，GUAN Xiaojing1，MEN Rongxin11. College of Marine Life Sciences，Ocean University of China，Qingdao 266003，ChinaReceived 23 October 2005; accepted 27 January 2006 |
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| 摘 要： | 1 IntroductionRibulose-1, 5-bisphosphatecarboxylase/oxyge-nase(RubisCO)isthem ostim portantenzymeforpho-tobiosynthesis. Itcatalyzesboth thereduction ofCO2and the oxygenolysisofribulose-1, 5-bisphosphate.Asthemostabundantproteinonearth (Ellis, 1979),RubisC…
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| 关 键 词： | 胶州湾 季节变化 基因多样性 基因序列 海洋生物 |
| 收稿时间： | 2005-10-23 |
| 修稿时间： | 2006-01-27 |
Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing |
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| LIU Yongjian,YANG Guanpin,GUAN Xiaojing,MEN Rongxin.Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing[J].Acta Oceanologica Sinica,2006,25(2):125-134. |
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| Authors: | LIU Yongjian YANG Guanpin GUAN Xiaojing and MEN Rongxin |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Ribulose-1, 5-bisphosphate carboxynase/ oxygenase large subunit gene (rbcL) of Jiaozhou Bay phytoplankton was amplified from spring, summer and autumn surface seawater DNAs and cloned respectively. About 50 clones were randomly selected from each library and sequenced. If identical amino acid sequences are considered as the same operational taxonomy unit (OTU), 61 OTUs are identified according to inferred amino acid sequences, among them, 21 from spring seawater, 15 from summer seawater and 25 from autumn seawater. Shannon index calculated from OTU abundances reflects the genetic diversity of a community. The indexes of spring, summer and autumn surface seawater phytoplankton are 2.69, 2.44 and 2.76 respectively, indicating that phytoplankton genetic diversity of autumn seawater is the richest. Seasonal variation of phytoplankton community is significant; the community compositions of three seasons are almost completely different except for the two OTUs shared by summer and autumn. Surface seawater phytoplankton communities are possibly metacommunities different spatially and temporally. |
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| Keywords: | phytoplankton ribulose-1 5-bisphosphate carboxynase/ oxygenase large subunit gene (rbcL) community structure seasonal variation |
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