| 预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响 |
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| 引用本文： | 姚敬元,刘瑀,李颖,王海霞.预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响[J].海洋学报(英文版),2016,35(6):1-6. |
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| 作者姓名： | 姚敬元 刘瑀 李颖 王海霞 |
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| 作者单位： | 环境科学与工程学院, 大连海事大学, 大连 116026, 中国,环境科学与工程学院, 大连海事大学, 大连 116026, 中国,环境信息研究所, 大连海事大学, 大连 116026, 中国,环境信息研究所, 大连海事大学, 大连 116026, 中国 |
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| 摘 要： | This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae(Isochrysis zhanjiangenisis).To identify the effects of sample preparation on theδ~(13)C value and the fatty acid composition,we examined eight types of pretreatment methods including:(a) drying the sample followed by direct methyl esterification using HCl-CH_3OH;(b) drying the sample followed by direct methyl esterification using H_2SO_4-CH_3OH;(c) drying the sample by ultrasonic extraction and methylesterification using HCl-CH_3OH;(d) drying the sample by ultrasonic extraction and methyl-esterification using H_2SO_4-CH_3OH;(e) fresh sample followed by direct methyl-esterification using HCl-CH_3OH;(f) fresh sample followed by direct methyl-esterification using H_2SO_4-CH_3OH;(g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH_3OH,and(h) fresh sample with ultrasonic extraction followed by methylesterification using H_2SO_4-CH_3 OH.The results show that the δ~(13)C values from Groups a–e,g and h fluctuated within 0.3‰,and the δ~(13)C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.
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| 关 键 词： | 碳稳定同位素分析 脂肪酸 海洋微藻 预处理 |
| 收稿时间： | 2015/6/23 0:00:00 |
| 修稿时间： | 2015/11/24 0:00:00 |
Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis |
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| YAO Jingyuan,LIU Yu,LI Ying and WANG Haixia.Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis[J].Acta Oceanologica Sinica,2016,35(6):1-6. |
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| Authors: | YAO Jingyuan LIU Yu LI Ying and WANG Haixia |
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| Institution: | 1.College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China2.Environmental Information Institute, Dalian Maritime University, Dalian 116026, China |
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| Abstract: | This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae (Isochrysis zhanjiangenisis). To identify the effects of sample preparation on the δ 13C value and the fatty acid composition, we examined eight types of pretreatment methods including: (a) drying the sample followed by direct methyl esterification using HCl-CH3OH; (b) drying the sample followed by direct methyl esterification using H2SO4-CH3OH; (c) drying the sample by ultrasonic extraction and methylesterification using HCl-CH3OH; (d) drying the sample by ultrasonic extraction and methyl-esterification using H2SO4-CH3OH; (e) fresh sample followed by direct methyl-esterification using HCl-CH3OH; (f) fresh sample followed by direct methyl-esterification using H2SO4-CH3OH; (g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH3OH, and (h) fresh sample with ultrasonic extraction followed by methylesterification using H2SO4-CH3OH. The results show that the δ 13C values from Groups a–e, g and h fluctuated within 0.3‰, and the δ 13C values of Group f were approximately 0.7‰ lower than the other seven groups. Therefore, the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios, and if necessary a correction should be applied. |
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| Keywords: | carbon stable isotope analysis fatty acid marine microalgae pretreatment |
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