| 致病性哈维氏弧菌:海洋寄生性纤毛虫的内共生细菌 |
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| 作者姓名： | QIAO Ying WANG Jun MAO Yong LIU Min CHEN Ruanni SU Yongquan KE Qiaozhen HAN Kunhuang ZHENG Weiqiang |
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| 作者单位： | State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China,College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China,College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China,College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China,College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China,State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China,State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China,State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China,State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China |
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| 摘 要： | 哈维氏弧菌被观察到与海洋寄生性纤毛虫刺激隐核虫具有内共生现象，其作为一种致病性细菌，可导致感染刺激隐核虫的大黄鱼产生严重的继发性细菌感染。我们通过16s宏测序技术对刺激隐核虫的细菌群落进行鉴定，并通过标准的细菌培养方法分离鉴定出哈维氏弧菌。通过透射电镜和荧光原位杂交方法，在刺激隐核虫的细胞胞质中，均观察到内共生的哈维氏弧菌的存在；而在其细胞核中，则没有相关信号存在。哈维氏弧菌与刺激隐核虫的相关关系以及内共生的哈维氏弧菌对刺激隐核虫的影响作用，仍需要深入进行研究。
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| 关 键 词： | 内共生细菌 原生动物 哈维氏弧菌 刺激隐核虫 16s宏测序 |
| 收稿时间： | 2016/4/29 0:00:00 |
Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans |
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| QIAO Ying,WANG Jun,MAO Yong,LIU Min,CHEN Ruanni,SU Yongquan,KE Qiaozhen,HAN Kunhuang,ZHENG Weiqiang.Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans[J].Acta Oceanologica Sinica,2017,36(10):115-119. |
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| Authors: | QIAO Ying WANG Jun MAO Yong LIU Min CHEN Ruanni SU Yongquan KE Qiaozhen HAN Kunhuang and ZHENG Weiqiang |
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| Institution: | 1.State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China;College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China2.College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China3.State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China |
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| Abstract: | Vibrio harveyi, known as a pathogenic bacterium caused severe secondary bacterial infections of the large yellow croaker Larimichthys crocea, was identified as an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans. Meta 16S sequencing method was used to identify the bacterial flora in C. irritans, and V. harveyi was isolated via culture-dependent method. Vibrio harveyi was observed in cytoplasm of C. irritans at the stage of tomont both by transmission electron microscopy and by Fluorescence in situ hybridization; no signal, however, was detected in nucleus area. The relationship between V. harveyi and C. irritans and the role of endosymbiotic V. harveyi in C. irritans merit further investigation. |
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| Keywords: | endosymbiotic bacterium protozoan Vibrio harveyi Cryptocaryon irritans Meta16S sequencing |
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