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Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis) |
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| Authors: | SHA Zhenxi REN Guocheng WANG Xianli WANG Na and CHEN Songlin |
| Institution: | 1. Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Qingdao 266071,China 2. Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Qingdao 266071,China;College of Biology,Shandong Normal University,Jinan 250014,China 3. Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Qingdao 266071,China;College of Marine Life,Ocean University of China,Qingdao 266003,China |
| Abstract: | A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,halfsmooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm3 bFGF(basic fibroblast growth factor),1 ng/cm3 LIF(leukemia inhibitory factor)and 50 mmol/dm32-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24 ℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE)after infection with lymphosystis disease virus(LCDV)in 2 d,Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus. |
| Keywords: | embryonic cell line half smooth tongue sole karyotype GFP LCDV CSEC |
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