| Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping |
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| 作者姓名： | DONG Shirui KONG Jie ZHANG Qingwen LIU Ping MENG Xianhong WANG Rucai |
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| 作者单位： | DONG Shirui~ 1，2，KONG Jie~ 2*，ZHANG Qingwen~2，LIU Ping~2，MENG Xianhong~2，WANG Rucai~11. College of Life Sciences and Technology，Ocean University of China，Qingdao 266003，China 2. Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources，Yellow Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Qingdao 266071，China |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金 |
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| 摘 要： | 1 IntroductionThe microsatellite DNA markers, which werealso called simple sequence repeats (SSR), are oneof the DNA markers developed in the late 1980s.The microsatellite DNA markers have several advan-tages over other molecular markers. For example,(1) …
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| 关 键 词： | 中国对虾 谱系示踪 微卫星DNA标记 基因分型 |
| 收稿时间： | 2006-03-17 |
| 修稿时间： | 2006-07-04 |
Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping |
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| DONG Shirui,KONG Jie,ZHANG Qingwen,LIU Ping,MENG Xianhong,WANG Rucai.Pedigree tracing of Fenneropenaeus chinensis by microsatellite DNA markers genotyping[J].Acta Oceanologica Sinica,2006,25(5):151-157. |
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| Authors: | DONG Shirui KONG Jie ZHANG Qingwen LIU Ping MENG Xianhong and WANG Rucai |
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| Institution: | College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China;Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Laboratory of Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Two microsatellite DNA loci were used to trace the pedigree structure of six families in the shrimp Fenneropenaeus chinensis. Four of the families were natural mating, and the others were mated by artificial insemination. Eleven alleles were acquired at two microsatellite DNA loci (locus RS0622 and locus EN0033) by investigating 145 offsprings and 11 parents. Five alleles were acquired from locus RS0622 and six from locus EN0033. As analyzed, the gene frequencies were between 0.024 1 and 0.493 1, the heterozygosity was 0.652 2 and 0.688 8, and the polymorphism information content (PIC) was 0.585 7 and 0.652 9 for the locus RS0622 and the locus EN0033, respectively. Twenty-three genotypes were detected and the genotypes of the losing parents were also inferred. The pedigrees of three F_1 and three F_2 generations were determined by matching the genotype at each locus. |
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| Keywords: | Fenneropenaeus chinensis microsatellite pedigree genotype |
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