| Bacterial diversity in the sediments collected from the Shikoku Basin |
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| 作者姓名： | MU Chunhu BAO Zhenmin CHEN Gang HU Jingjie HAO Lujiang QI Zizhong LI Guangxue |
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| 作者单位： | [1]LaboratoryofMarineGeneticsandBreedings（MGB）,DivisionofLifeScienceandTechnology,OceanUniversityofChina,Qingdao266003,China [2]CollegeofMarineGeosciences,OceanUniversityofChina,Qingdao266003,China |
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| 摘 要： | Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups: Proteobacteria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation.
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| 关 键 词： | 细菌差异 深海沉积 四国湾 太平洋西北部 |
| 收稿时间： | 2004/12/11 0:00:00 |
| 修稿时间： | 2005/5/20 0:00:00 |
Bacterial diversity in the sediments collected from the Shikoku Basin |
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| MU Chunhu,BAO Zhenmin,CHEN Gang,HU Jingjie,HAO Lujiang,QI Zizhong,LI Guangxue.Bacterial diversity in the sediments collected from the Shikoku Basin[J].Acta Oceanologica Sinica,2005,24(3):114-121. |
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| Authors: | MU Chunhu BAO Zhenmin CHEN Gang HU Jingjie HAO Lujiang QI Zizhong and LI Guangxue |
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| Institution: | 1.Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China2.College of Marine Geosciences, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups: Proteobac-teria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation. |
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| Keywords: | bacteria diversity deep-sea sediment Shikoku Basin Northwest Pacific Ocean |
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