| Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers |
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| 作者姓名： | WANG Wei YOU Feng XU Jianhe SUN Wei ZHU Xiangping GAO Tianxiang ZHANG Peijun |
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| 作者单位： | WANG Wei（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;College of Life Sciences and Technology,Ocean University of China,Qingdao 266003,China） ;
YOU Feng（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ;
XU Jianhe（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ;
SUN Wei（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ;
ZHU Xiangping（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ;
GAO Tianxiang（College of Life Sciences and Technology,Ocean University of China,Qingdao 266003,China） ;
ZHANG Peijun（Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China） ; |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，上海市自然科学基金 |
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| 摘 要： | Twenty microsatellite markers （ Pol, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, kop6, kop7, kop8, kop12, kop15, kop18, kop21, kop22, kop23, kop26, Po-str1 ） were used to assess the meiogynogenetic and mitogynogenetic stocks of the lefteyed flounder, Paralichthys olivaceus, which were derived from single pair crossing. Twelve of the 20 loci utilized showed heterozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder. Microsatellitecentromere map distance, calculated under the assumption of complete interference, ranged from 15. 8 cM for kop22 to 50 cM for Po13, Po56 and Po89. Excluding the kop22, the heterozygosities of the rest of the loci were close to 100% , suggesting the occurrence of near complete interference on the chromosome arms that carried these loci. In the mitogynogenetic diploid flounder, each individual showed exact homozygousity and the segregation profiles did not deviate from the Mendelian 1：1 pattern. The results indicated that there was no lethal gene linked with the loci analyzed. Such high interference accounted for the high recombination rates and large map distances. The Po13 and Po56 loci, Po91 and kopl8 loci, kopl5 and kop21 loci are tightly linked on the same chromosome arm in pairs.
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| 关 键 词： | 牙鲆 遗传分析 着丝粒 雌核发育 微卫星标记 |
| 收稿时间： | 4/2/2007 12:00:00 AM |
| 修稿时间： | 2007/8/10 0:00:00 |
Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers |
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| WANG Wei,YOU Feng,XU Jianhe,SUN Wei,ZHU Xiangping,GAO Tianxiang,ZHANG Peijun.Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers[J].Acta Oceanologica Sinica,2008,27(2):149-156. |
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| Authors: | WANG Wei YOU Feng XU Jianhe SUN Wei ZHU Xiangping GAO Tianxiang and ZHANG Peijun |
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| Institution: | 1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China3.College of Life Sciences and Technology, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Twenty microsatellite markers (Po1,Po13 ,Po33,Po35,Po42,Po48,Po56,Po89,Po91,kopr ,kop7 ,kop8,kop12,kop15, kopl8,kop21,kop22,kop23,kop26,Po-strl ) were used to assess the meiogynogenetic and mitogynogenetic stocks of the left-eyed flounder,Paralichthys olivaceus,which were derived from single pair crossing.Twelve of the 20 loci utilized showed het-erozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder.Microsatellite-centromere map distance,calculated under the assumption of complete interference,ranged from 15.8 cM for kop22 to 50 cM for Po13,Po56 and Po89.Excluding the kop22,the heterozygosities of the rest of the loci were close to 100%,suggesting the occur-rence of near complete interference on the chromosome arms that carried these loci.In the mitogynogenetic diploid flounder,each individual showed exact homozyguusity and the segregation profiles did not deviate from the Mendelian 1:1 pattern.The results in-dicated that there was no lethal gene linked with the loci analyzed.Such high interference accounted for the high recombination rates and large map distances.The Pol3 and Po56 loci,Po91 and kop18 loci,kop15 and kop21 loci are tightly linked on the same chromosome arm in pairs. |
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| Keywords: | Paralichthys olivaceus meiogynogenesis mitogynogenesis microsatellite-centromere mapping |
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