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富油微藻富碳培养下油脂积累及固碳研究
引用本文:王帅,郑立,韩笑天,杨佰娟,李景喜,孙承君.富油微藻富碳培养下油脂积累及固碳研究[J].海洋学报(英文版),2018,37(2):119-126.
作者姓名:王帅  郑立  韩笑天  杨佰娟  李景喜  孙承君
作者单位:海洋活性物质与现代分析技术国家海洋局重点实验室 国家海洋局第一海洋研究所, 山东 青岛 266061,海洋活性物质与现代分析技术国家海洋局重点实验室 国家海洋局第一海洋研究所, 山东 青岛 266061;青岛海洋科学与技术国家实验室 海洋生态与环境科学功能实验室, 山东 青岛 266071,海洋生态与环境科学重点实验室 中科院海洋所, 山东 青岛 266071,海洋活性物质与现代分析技术国家海洋局重点实验室 国家海洋局第一海洋研究所, 山东 青岛 266061,海洋活性物质与现代分析技术国家海洋局重点实验室 国家海洋局第一海洋研究所, 山东 青岛 266061,海洋活性物质与现代分析技术国家海洋局重点实验室 国家海洋局第一海洋研究所, 山东 青岛 266061
基金项目:The Basic Scientific Fund for National Public Research Institutes of China under contract Nos 2017Q09 and 2016Q02; the National Natural Science Foundation of China under contract No. 41776176; the National Key Research and Development Program of China under contract No. 2017YFC1404604; the Shandong Provincial Natural Science Foundation under contract No. ZR2015PD003; the 2012 Taishan Scholar.
摘    要:微藻固碳是一种新型节能减排技术,具有长期可持续发展的潜力。本文对两株富油微藻(球等鞭金藻和微拟球藻)进行了富碳培养下生长特性及中性脂积累特性的研究。两株富油微藻的最佳培养条件为10%CO2浓度和f培养基。本研究对两株富油微藻的最大生物量产率、总脂含量、最大油脂产率、微藻的C含量和CO2固定率进行了测定。球等鞭金藻的各参数指标分别为:142.42±4.58g/(m2·d),39.95%±0.77%,84.47±1.56g/(m2·d),45.98%±1.75%和33.74±1.65g/(m2·d)。微拟球藻的各参数指标分别为:149.92±1.80g/(m2·d),37.91%±0.58%,89.90±1.98g/(m2·d),46.88%±2.01%和34.08±1.32g/(m2·d)。实验结果显示,两株海洋微藻均属于高固碳优良藻株,适合应用于微藻烟气减排技术开发,具备用于海洋生物质能耦合CO2减排开发的潜力。

关 键 词:球等鞭金藻  微拟球藻  CO2富碳培养  中性脂  生物柴油  跑道池
收稿时间:2017/5/4 0:00:00
修稿时间:2011/6/14 0:00:00

Lipid accumulation and CO2 utilization of two marine oil-rich microalgal strains in response to CO2 aeration
WANG Shuai,ZHENG Li,HAN Xiaotian,YANG Baijuan,LI Jingxi and SUN Chengjun.Lipid accumulation and CO2 utilization of two marine oil-rich microalgal strains in response to CO2 aeration[J].Acta Oceanologica Sinica,2018,37(2):119-126.
Authors:WANG Shuai  ZHENG Li  HAN Xiaotian  YANG Baijuan  LI Jingxi and SUN Chengjun
Institution:Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China,Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China,Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China,Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China and Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Abstract:Biological CO2 sequestration by microalgae is a promising and environmentally friendly technology applied to sequester CO2. The characteristics of neutral lipid accumulation by two marine oil-rich microalgal strains, namely, Isochrysis galbana and Nannochloropsis sp., through CO2 enrichment cultivation were investigated in this study. The optimum culture conditions of the two microalgal strains are 10% CO2 and f medium. The maximum biomass productivity, total lipid content, maximum lipid productivity, carbon content, and CO2 fixation ability of the two microalgal strains were obtained. The corresponding parameters of the two strains were as follows: ((142.42±4.58) g/(m2·d), (149.92±1.80) g/(m2·d)), ((39.95±0.77)%, (37.91±0.58)%), ((84.47±1.56) g/(m2·d), (89.90±1.98) g/(m2·d)), ((45.98±1.75)%, (46.88±2.01)%), and ((33.74±1.65) g/(m2·d), (34.08±1.32) g/(m2·d)). Results indicated that the two marine microalgal strains with high CO2 fixation ability are potential strains for marine biodiesel development coupled with CO2 emission reduction.
Keywords:Isochrysis galbana  Nannochloropsis sp    CO2 enrichment cultivation  neutral lipid  biodiesel  open raceway pond
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