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中国重点海水浴场中粪肠球菌和主要肠道病毒的调查研究
引用本文:樊景凤,明红霞,吴立军,梁玉波,李雪.中国重点海水浴场中粪肠球菌和主要肠道病毒的调查研究[J].海洋通报(英文版),2011,13(2):72-82.
作者姓名:樊景凤  明红霞  吴立军  梁玉波  李雪
作者单位:1. 国家海洋环境监测中心,辽宁大连,116023
2. 南开大学,环境科学与工程学院,国家教育部重点实验室,天津300071
3. 中科院武汉病毒研究所,湖北武汉,430071
4. 国家海洋信息中心,天津,300171
摘    要:本文调查了中国十大典型海水浴场,旨在监测人类肠道病毒在海水浴场的发生情况,并且找出表面海水中肠道病毒的存在与传统的粪便污染指示物一粪大肠菌群是否存在关系,如果存在,是什么关系。分别采用常规的平板计数法和RT-PCR技术对粪大肠菌群和人类致病性的主要肠道病毒(甲肝病毒、轮状病毒、脊髓灰质炎病毒)进行了检测。结果显示,在2...

关 键 词:粪便污染:粪大肠菌群  肠道病毒  传统指示生物  海水浴场

Investigation of fecal coliform and typical enteric virus in representative beaches of China
FAN Jing-feng,MING Hong-xia,WU Li-jun,LIANG Yu-bo,LI Xue.Investigation of fecal coliform and typical enteric virus in representative beaches of China[J].Marina Science Bulletin,2011,13(2):72-82.
Authors:FAN Jing-feng  MING Hong-xia  WU Li-jun  LIANG Yu-bo  LI Xue
Institution:1. National Marine Environmental Monitoring Center, Linghe street 42, Dalian 116023, Liaoning Provine, China; 2. College of Environmental Science and Engineering, Key Laboratory of Pollution and Environmental Criteria Ministry of Education, Nankai University, Tianjin 300071, China; 3. Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, Hubei Province, China; 4. National Marine Data and Information Service, Tianjin 300171, China
Abstract:Through investigating ten recreational marine beaches in China, we aimed to detect the occurrence of human enteric viruses in coastal bathing beaches and find a correlationship, if any, between the presence of enteric viruses in surface seawater and the concentrations of fecal coliforms, the conventional indicator of fecal pollution. In this study, twenty seawater samples were assayed for fecal coliforms and human pathogenic enteric viruses (hepatitis A viruses, rotaviruses, polioviruses) analysis. Enteric viruses were detected by RT-PCR, in 20 sample sites, 5%, 40%, 40% were positive for the presence of human hepatitis A viruses, rotaviruses, polioviruses, respectively. Seven of 20 sites are suffering from severe fecal contamination, based on traditional plate counts of fecal coliform outnumbering the established thresholds for recreation. Additionally, statistical analysis presented that no correlation was found between bacterial indicators and viruses in surface seawaters. The data confirmed that indicator bacteria in water are not reflective of the presence of enteric viruses in marine waters. Thus, current recreational water quality standards of both bacterial and viral indices should be reevaluated.
Keywords:fecal pollution  fecal coliform  enteric viruses  traditional indicator  public beaches
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