| 从培养的微藻中初步筛选抗菌和抗肿瘤活性物质 |
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| 引用本文： | 邓伟,王雪青,宋文军.从培养的微藻中初步筛选抗菌和抗肿瘤活性物质[J].海洋通报(英文版),2011,13(1):60-70. |
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| 作者姓名： | 邓伟 王雪青 宋文军 |
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| 作者单位： | 天津食品与生物技术重点实验室,天津商业大学,生物技术与食品科学学院,天津,300134 |
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| 基金项目： | This work is supported by the Natural Science Foundation of Tianjin （Grant No. 08JCZDJC16600）. We also would like to thank Key Development Programs of Tianjin in Science and Technology （Grant No. 06YFGZNC04200）. |
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| 摘 要： | 从8种微藻中制备水溶和脂溶性的提取物,用来筛选抗菌和抗肿瘤活性.抗菌活性是用抑菌豳法(细菌用金黄色葡萄球菌,枯草芽孢杆菌和大肠杆菌为指示菌,真菌用黑曲霉和产黄青霉为指示菌).实验选用的所有的微藻均有不同程度的抗一种或多种菌活性,抗金黄色葡萄球菌微藻提取物中的57.14%有抗耐甲氧西林金黄色葡萄球菌活性,采用MTT法体外...
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| 关 键 词： | 微藻 筛选 抗菌 抗肿瘤 |
Preliminary screening of antimicrobial and antitumor activities from cultivated microalgaes |
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| DENG Wei,WANG Xue-qing,SONG Wen-jun.Preliminary screening of antimicrobial and antitumor activities from cultivated microalgaes[J].Marina Science Bulletin,2011,13(1):60-70. |
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| Authors: | DENG Wei WANG Xue-qing SONG Wen-jun |
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| Institution: | The Tianjin Key Laboratory of Food and Biotechnology; Tianjin University of commerce, College of Biotechnology and Food Science, Tianjin, 300134, China |
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| Abstract: | Hydrophilic and lipophilic extracts were prepared from 8 microalgal strains, and screened for antimicrebial and antitumor activities. Antimicrobial activity was determined by observing bacterial (S. aureus, Bacillus subtilis and Escherichia coli) and fungal(Aspergillus niger and Penicillium chrysogenum) growth inhibition. All the microalgae had different degrees of antimicrobial activity against one or more microbe - tested, and 56.47% of the extracts showing the anti-S.aureus activity exhibited the antibacterial activity against (MRSA). Cytotoxic activities were measured in vitro against human cancer cell lines HeLa by the MTT assay. Most of these extracts showed potent activity against the growth of the tumor cells, especially the intracellular lipophilic extracts from Isochrysis galbana Parke 3011 and Isochrysis galbana Parke H29, which exhibited strong antitumor activity against HeLa cell lines. The overall results of this study indicate that the extracts from microalgae represent a potential sources of medicine for the treatment of infectious and cancer diseases. |
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| Keywords: | microalgae screening antimicrobial antitumor |
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