| 雨生红球藻中crtO基因存在3个5’上游侧翼序列 |
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| 引用本文： | 高政权,孟春晓,叶乃好.雨生红球藻中crtO基因存在3个5’上游侧翼序列[J].海洋通报(英文版),2010,12(1):59-64. |
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| 作者姓名： | 高政权 孟春晓 叶乃好 |
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| 作者单位： | 1. 山东理工大学生命科学学院,山东,淄博,255049
2. 中国水产科学院黄海水产研究所,山东,青岛,266071 |
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| 基金项目： | The research were supported by the National Natural Science Foundation，Natural Science Foundation of Shandong University of Technology，Start-up Foundation for Ph.D of Shandong University of Technology，National Key Technology R&D program，Foundation of State Commonweal Institute |
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| 摘 要： | 在本研究中，我们首次通过基因组上游第一次步移克隆到了雨生红球藻中crtO基因的3个不同大小的5’上游侧翼序列1．1kb，1．9kb和2．2kb。利用生物信息学方法分别预测并比较了这3个不同大小的5’上游侧翼序列，结果发现，三者具有与高等植物相类似的顺式作用元件，如脱落酸反应元件（ABRE）、干燥或低温反应元件（DRE／C-repeat）、几种光反应元件（G1-box,GAG-motif，l-boxand ATC—motif）、热激反应元件（HSE）、机械伤害反应元件（WUN-motif）、生长素反应元件（TGA-element）、茉莉酸甲酯反应元件（TGACG-element）和反式作用因子MYB蛋白的结合位点（MBSandMRE）等等，但是三者并不具有典型的TATA框和CCAAT框。上述研究预示了雨生红球藻虾青素合成中crtO基因调控方式的多样化。
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| 关 键 词： | 虾青素 顺式作用元件 5’上游侧翼学列 雨生红球藻 crtO |
Three5'-flanking Regions of crtO Encoding β-carotene Oxygenase in Haematococcus pluvialis |
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| GAO Zheng-quan,MENG Chun-xiao,YE Nai-hao.Three5''-flanking Regions of crtO Encoding β-carotene Oxygenase in Haematococcus pluvialis[J].Marina Science Bulletin,2010,12(1):59-64. |
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| Authors: | GAO Zheng-quan MENG Chun-xiao YE Nai-hao |
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| Institution: | 1. School of Life Sciences, Shandong University of Technology, Zibo 255049, Shandong, China 2. Yellow Sea Fishery Research Institute, Qingdao 266071, Shandong, China) |
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| Abstract: | Three separate 5'-flanking regions (1.1 kb, 1.9 kb and 2.2 kb) of crtO were cloned through walking upstream. Results of sequence analysis show that three 5'-flanking regions of crtO might have some similar putative cis-acting elements such as ABA (abscisic acid)-responsive element (ABRE), C-repeat/dehydration responsive element (C-repeat/DRE), light-responsive element (G-box, GAG-motif, I-box and ATC-motif), wound-responsive element (WUN-motif), auxin-responsive element (TGA-element), MeJA-responsive element (TGACG-element) and MYB binding site (MBS), except for typical TATA box or CCAAT box. These findings might mean diversiform regulatory patterns of crtO being in astaxanthin biosynthesis of Haematococcus pluvialis. |
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| Keywords: | crtO astaxanthin cis-acting elements crtO 5'-flanking region Haematococcus pluvialis |
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