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几种化学物质对两种牡蛎幼虫附着和变态的诱导
引用本文:方琦,林笔水,方永强.几种化学物质对两种牡蛎幼虫附着和变态的诱导[J].台湾海峡,2001,20(1):20-26.
作者姓名:方琦  林笔水  方永强
作者单位:国家海洋局第三海洋研究所,
基金项目:福建省重中之重资助项目
摘    要:本文研究5种神经递质和1种神经肽对两种牡蛎幼虫附着和变态的诱导效应。结果表明:L-多巴对牡蛎幼虫附着行为的诱导快速而有效,浓度10μmol/dm^3,2h内有附着行为的幼虫约占50%,并使之正常变态,肾上腺素、去甲肾上腺素和L-多巴均能有效地诱导两种牡蛎幼虫变态,它们的最适诱导浓度分别为50、50和5μmol/dm^3,诱导变态率分别达97.1%、90.6%和70.5%(长牡蛎),74.9%、74.3%和37.2%(僧帽牡蛎);多巴胺的诱导变态效果不显著,而γ-氨基丁酸和5-羟色胺不能有效诱导变态。两种牡蛎幼虫对上述有效诱导药物的反应,长牡蛎优于僧帽牡蛎。肾上腺素诱导长牡蛎,最适处理时机在眼点幼虫后期,持续处理时间为2h之内。

关 键 词:肾上腺素  化学诱导  附着  长牡蛎  僧帽牡蛎  幼虫  L-多巴
文章编号:1000-8160(2001)01-0020-07

Induction of larval settlement and metamorphosis of two oysters Crassostrea gigas and Ostrea cucullata by some chemicals
FANG Qi,LIN Bi-shui,FANG Yong-qiang.Induction of larval settlement and metamorphosis of two oysters Crassostrea gigas and Ostrea cucullata by some chemicals[J].Journal of Oceanography In Taiwan Strait,2001,20(1):20-26.
Authors:FANG Qi  LIN Bi-shui  FANG Yong-qiang
Abstract:Five neurotransmitters and a neuropeptide were examined for induction of settlement and metamorphosis in larvae of two oysters Crassostrea gigas and Ostrea cucullata.The results indicated that L-3,4-dihydroxyphenylalanine(L-DOPA)inductive settlement behavior was rapidly effective with about 50% larvae at concentration 10μmol/dm3 within 2h,subseequently made them metamorphosis.Epinephrine (EPI) norepinephrine (NE) and L-DOPA were effctive to induce larvae metamorphosis,at optimal inductive concentration 50,50 and 5μmol/dm3 respectivitly,given 97.1%,90.5% and 70.5% metamorphosis for C.gigas and 74.9%,74.3% and 37.2% for O.cucullata.Incontrast,γ-aminobutyric acid and 5-hydroxytryptamine were ineffective.Responses of C.gigas metamorphosis to chemical induction was more active than O.cucullata.By examining EPI to C.gigas,it was indicated that inductive occasion was at post eyed-larvea for exposure treatment within 2h.
Keywords:Chemical  induction  settlement  metamorphosis  Crassostrea  gigas  Ostrea  cucullata
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