| 不同浓度苯并（a）芘对杂色鲍抗氧化酶活性的影响 |
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| 引用本文： | 刘洁,林智勇,王克坚.不同浓度苯并（a）芘对杂色鲍抗氧化酶活性的影响[J].台湾海峡,2014(4):486-491. |
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| 作者姓名： | 刘洁 林智勇 王克坚 |
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| 作者单位： | 厦门大学近海海洋环境科学国家重点实验室,福建 厦门,361005 |
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| 基金项目： | 教育部长江学者和创新团队发展计划资助项目 |
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| 摘 要： | 为阐释不同浓度苯并（a）芘B（a）P]对杂色鲍（Haliotis diversicolor）的毒性效应,以0.02、0.04和0.08 mg/dm^33个质量浓度的B（a）P对杂色鲍进行水体暴露胁迫,7d后检测肌肉、外套膜、鳃、性腺、肾和肝胰腺的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）和谷胱甘肽过氧化物酶（GPx）活性及还原型谷胱甘肽（GSH）含量.结果显示：0.02 mg/dm^3的B（a）P暴露对杂色鲍抗氧化系统的影响不明显,而0.04、0.08 mg/dm^3的B（a）P则显著抑制了SOD、CAT和GPx酶活性以及GSH含量,同时对GST酶活性具有显著的诱导作用;实验还发现,不同组织抗氧化酶活性对B（a）P胁迫的敏感性响应存在较大差异,其中鳃、肾脏和肝胰腺的敏感性响应明显高于肌肉和外套膜.上述结果表明,苯并（a）芘暴露对杂色鲍抗氧化酶活性的影响具有明显的剂量-效应关系及组织差异性,杂色鲍的鳃、肾脏或者肝胰腺的抗氧化酶响应多环芳香烃胁迫的更敏感,可作为B（a）P污染的生物标志物.
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| 关 键 词： | 海洋生物学 杂色鲍 苯并(a)芘 抗氧化酶活性 |
Effects of different concentrations of benzo(a)pyrene on antioxidant enzymes in gastropod abalone,Haliotis diversicolor |
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| LIU Jie,LIN Zhi-yong,WANG Ke-jian.Effects of different concentrations of benzo(a)pyrene on antioxidant enzymes in gastropod abalone,Haliotis diversicolor[J].Journal of Oceanography In Taiwan Strait,2014(4):486-491. |
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| Authors: | LIU Jie LIN Zhi-yong WANG Ke-jian |
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| Institution: | (State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China) |
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| Abstract: | To reveal the ecotoxicological effects of benzo（a） pyreneB（a） P]on gastropod abalone,Haliotis diversicolor,the effects of different concentrations of B（a） P（0.02,0.04 and 0.08 mg/dm^3） were studied on antioxidant defense system of H.diversicolor.The results showed that the antioxidant enzymes activities showed little difference for the animals exposed to 0.02 mg/dm^3B（a） P compared with the control.In 0.04 mg/dm^3 and 0.08 mg/dm^3 groups,the activities of SOD,CAT,GPx and the contents of GSH were significant inhibited by B（a） P exposure,while the GST activities were significantly induced.The activities of antioxidant enzymes were also significantly different among the tissues,suggesting the exist of remarkable different physiological functions and responses to B（a） P content.In summary,the antioxidant enzymes activities of exposure to B（a） P exhibit an effects of concentration-dependent and are tissue specified in H.diversicolor and the antioxidant enzymes system of the gills,kidney and hepatopancreas are more sensitive.Thus it can be used as pollution biomarkers for PAHs stress. |
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| Keywords: | marine biology Haliotis diversicolor benzo(a)pyrene antioxidant enzymes |
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