| 几种养殖鲍同工酶生化遗传的比较研究 |
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| 引用本文： | 柯才焕,陈晓佳,周时强,王志勇,王艺磊.几种养殖鲍同工酶生化遗传的比较研究[J].台湾海峡,2003,22(2):173-179,T002. |
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| 作者姓名： | 柯才焕 陈晓佳 周时强 王志勇 王艺磊 |
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| 作者单位： | 1. 厦门大学海洋系、亚热带海洋研究所,福建,厦门,361005
2. 集美大学水产生物技术研究所,福建,厦门,361021 |
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| 基金项目： | 国家高技术研究发展计划(863计划)课题(2001AA621080);福建省自然科学基金资助项目(9820002) |
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| 摘 要： | 运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍(皱纹盘鲍、盘鲍及九孔鲍)12种同工酶(LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT)26个基因座位进行了分析．结果表明：EST、PGM可以作为区分三种(亚种)鲍的生化遗传标记；在所分析的位点中，未发现多态现象，表明三种养殖鲍杂合子缺失严重，其主要原因可能在于哑基因、近交与自然选择。
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| 关 键 词： | 皱纹盘鲍 盘鲍 九孔鲍 聚丙烯酰胺凝胶电泳 生化遗传学 同工酶 海洋水产 |
| 文章编号： | 1000-8160(2003)02-0173-07 |
Biochemical genetic comparisons of isozymes on cultured abalones in China |
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| KE Cai huan ,CHEN Xiao jia ,ZHOU Shi qiang ,WANG Zhi yong ,WANG Yi lei.Biochemical genetic comparisons of isozymes on cultured abalones in China[J].Journal of Oceanography In Taiwan Strait,2003,22(2):173-179,T002. |
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| Authors: | KE Cai huan CHEN Xiao jia ZHOU Shi qiang WANG Zhi yong WANG Yi lei |
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| Institution: | KE Cai huan 1,CHEN Xiao jia 1,ZHOU Shi qiang 1,WANG Zhi yong 2,WANG Yi lei |
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| Abstract: | Twelve isoenzymes ( LDH, ME, MDH, GPI, PGM, IDH, IDDH, SOD, EST, ODH, ADH, AAT) extracted from three species of cultured abalones ( Haliotis discus hannai, H. diversicolor supertexta, H. discus discus ) in China were determined by polyacrylamide gel electrophoresis (PAGE), twenty six genic loci were tested but no polymorphic loci was detected. The result shows: 1) EST and PGM can be used as biochemical genetic markers to identify three species of abalone. 2) Among the loci tested, no polymorphic loci was detected, which means that there is serious loss of heterozygotes. The main reason may be null allele, close breeding and darwin selection. |
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| Keywords: | marine fishery biochemical genetics polyacrylamide gel electrophoresis Haliotis discus hannai Haliotis discus discus Haliotis diversicolor supertexta isozyme |
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