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| 东山九孔鲍病毒病病毒的分离观察 | |
| 引用本文： | 方莹,黄印尧,颜江华,颜青,吴文忠,倪子绵.东山九孔鲍病毒病病毒的分离观察[J].台湾海峡,2002,21(2):199-202,T003. |
| 作者姓名： | 方莹 黄印尧 颜江华 颜青 吴文忠 倪子绵 |
| 作者单位： | 1. 厦门大学生命科学学院抗癌研究中心,福建,厦门,361005 2. 厦门出入境检验检疫局,福建,厦门,361012 3. 厦门大学化学化工学院电镜室,福建,厦门,361005 |
| 摘 要： | 运用电镜负染和超薄切片技术，在福建东山患病的九孔鲍的肾组织细胞，肝细胞和肠上皮细胞中可见有大小为100nm的具包囊的六角形病毒颗粒，病鲍组织匀浆后，经蔗糖分步超速离心，RNA酶及DNA酶消化去除组织内DNA，RNA后，获得纯化的病毒颗粒，又经蛋白酶K消化后得到病毒的核酸，以DNaseⅠ或RNaseA分别消化，经1%（m/n)的琼脂糖凝胶电泳证实该病毒为DNA病毒。 |
| 关 键 词： | 九孔鲍 病毒 分离 电镜 超薄切片技术 肝细胞 |
| 文章编号： | 1000-8160(2002)02-0199-04 |
Isolation and observation of "virus disease" virus of abalone in Dongshan,Fujian |
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| FANG Ying ,HUANG Yin yao ,YAN Jiang hua ,YAN Qing ,WU Wen zhong ,NI Zi mian.Isolation and observation of "virus disease" virus of abalone in Dongshan,Fujian[J].Journal of Oceanography In Taiwan Strait,2002,21(2):199-202,T003. | |
| Authors: | FANG Ying HUANG Yin yao YAN Jiang hua YAN Qing WU Wen zhong NI Zi mian |
| Institution: | FANG Ying 1,HUANG Yin yao 2,YAN Jiang hua 1,YAN Qing 1,WU Wen zhong 2,NI Zi mian 3 |
| Abstract: | Using ultramicrotomy technique and electron microscopy technique combined with negative staining method, hexagal particles containing capsule in size around 100nm were observed in renal cell, liver cell and enteron cell of diseased abalone sampled from Dongshan, Fujian. Homogenized tissue of diseased abalone was super centrifugalized in ladder sucrose medium, followed by the digestion of DNA and RNA in tissue using DNase and RNase. Pure virus particles were then obtained. Nucleic acid of the virus was achieved by digesting the virus particles using proteinase K. Purified nucleic acid was digesting by DNaseI and RNaseA, respectively. This virus was ascertained to be DNA virus by 1%( m/m ) agarose electrophoesis. |
| Keywords: | Haliotis diversicolor virus isolation |
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