| 贻贝超氧化物歧化酶的分离纯化和部分性质 |
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| 引用本文： | 肖湘,俞丽君,郑扬明,张尔贤.贻贝超氧化物歧化酶的分离纯化和部分性质[J].台湾海峡,1999,18(2):177-180,T001. |
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| 作者姓名： | 肖湘 俞丽君 郑扬明 张尔贤 |
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| 作者单位： | 汕头大学理学院生物系，汕头 515063 |
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| 摘 要： | 采用热变性－硫酸铵分级沉淀－Ｓｅｐｈａｄｅｘ Ｇ－１００和ＤＥ５２柱层析的方法从贻贝中分离纯化铜锌超氧化物歧化酶，并对其部分性质进行分析鉴定。结果该酶的比活力为９４５３．３Ｕ／ｍｇ（ｐｒｏｔｅｉｎ），提纯倍数为６０９．９，对ＫＣＮ和Ｈ２Ｏ敏感，Ｔ氏液和ＳＤＳ对酶活性没影响，对热较稳定。
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| 关 键 词： | 贻贝 超氧化物歧化酶 纯化 性质 |
Separation and purification of superoxide dismutase containing copper and zinc from Mytilus and analysis of its character |
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| Xiao Xiang,Yu Lijun,Zheng Yangming,Zhang Erxian.Separation and purification of superoxide dismutase containing copper and zinc from Mytilus and analysis of its character[J].Journal of Oceanography In Taiwan Strait,1999,18(2):177-180,T001. |
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| Authors: | Xiao Xiang Yu Lijun Zheng Yangming Zhang Erxian |
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| Abstract: | A method of heating denaturation, precipitation with ammonium sulphate and passing Sephadex G-100 and DE 52 chromatography colums was used to separate and purify superox ide dismutase containing copper and zinc(Cu,Zn-SOD) from Mytilus,and part of its charac ters were analyzed. The results showed that the specific activity of the enzyme was 9 453. 3 U/mg, and its multiple purified was 609. 9. The enzyme was sensitive to KCN and H_2O_2,and not sensitive to Tsuchihashi solution and SDS. It was stable in heat condition. Its ultraviolet absorption maxinum was at 270 nm. It showed three bands by polyacrylamide gel elec trophoresis. The enzymy subunit molecular weight was 16 500u. |
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| Keywords: | Mytilus superoxide dismutase purify character |
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